@Article{biocell.2022.021359,
AUTHOR = {BINFEN HOU, LI ZHAO, T IANHAO ZHAO, MINGMING YANG, WANWAN ZHU, XIAODONG CHEN, XIQUAN KE, ZHENZENG MA, LIN GU, MENG WANG, MIN DENG},
TITLE = {Chrysophanol inhibits the progression of gastric cancer by activating nod-like receptor protein-3},
JOURNAL = {BIOCELL},
VOLUME = {47},
YEAR = {2023},
NUMBER = {1},
PAGES = {175--186},
URL = {http://www.techscience.com/biocell/v47n1/49921},
ISSN = {1667-5746},
ABSTRACT = {<b>Aim:</b> Gastric cancer (GC) is one of the most common malignant tumors. Chrysophanol has been reported to
possess antitumor effects on a variety of cancers; however, its role in GC remains unclear. This study aimed to investigate
the effects of chrysophanol on the proliferation, pyroptosis, migration, and invasion of GC cells. <b>Methods:</b> Human GC
cell lines MKN 28 and AGS cells were treated with different concentrations of chrysophanol, then cell proliferation,
migration, invasion and pyroptosis were determined by CCK-8, colony-forming assay, wound healing assay, Transwell
assay, and flow cytometry. Cell migration and invasion were reassessed in these transfected cells following the
transfection of nod-like receptor protein-3 (NLRP3) siRNA in MKN 28 and AGS cells. To examine the downstream
signaling pathway of the NLRP3 signaling pathway, NLRP3, caspase-1, gasdermin-D, interleukin (IL)-1β, and IL-18
were detected by quantitative real-time-polymerase chain reaction or western blotting. <b>Results: </b>Chrysophanol
inhibited the proliferation of GC cells, caused pyroptosis, inhibited cell migration and invasion, and increased the
expression of NLRP3 inflammasomes in GC cells. Knockdown of NLRP3 inhibited the effects of chrysophanol on
proliferation, pyroptosis, migration, and invasion of GC cells. Chrysophanol plays an anticancer role by enhancing
NLRP3. <b>Conclusions:</b> Chrysophanol exerts anti-neoplastic effects in vitro in GC cells by modulating NLRP3, thus
highlighting its therapeutic potential in GC.},
DOI = {10.32604/biocell.2022.021359}
}