@Article{biocell.2022.022477,
AUTHOR = {JINXIAN LIU, JINGFANG FENG, CHANG ZHANG, YONGJUAN REN, WEIHUA SU, GUANGHENG WU, XIANYU FU, NING HUANG, YOUXIONG QUE, HUI LING, JUN LUO},
TITLE = {Overexpression of a sugarcane<i> ScCaM</i> gene negatively regulates salinity and drought stress responses in transgenic <i>Arabidopsis thaliana</i>},
JOURNAL = {BIOCELL},
VOLUME = {47},
YEAR = {2023},
NUMBER = {1},
PAGES = {215--225},
URL = {http://www.techscience.com/biocell/v47n1/49922},
ISSN = {1667-5746},
ABSTRACT = {Calmodulin (CaM) proteins play a key role in signal transduction under various stresses. In the present study,
the effects of a sugarcane <i>ScCaM</i> gene (NCBI accession number: GQ246454) on drought and salt stress tolerance in
transgenic <i>Arabidopsis thaliana</i> and <i>Escherichia coli</i> cells were evaluated. The results demonstrated a significant
negative role of <i>ScCaM</i> in the drought and salt stress tolerance of transgenic lines of <i>A. thaliana</i>, as indicated by the
phenotypes. In addition, the expression of <i>AtP5CS</i> and <i>AtRD29A</i>, two genes tightly related to stress resistance, was
significantly lower in the overexpression lines than in the wild type. The growth of <i>E. coli</i> BL21 cells expressing
<i>ScCaM</i> showed weaker tolerance under mannitol and NaCl stress. Taken together, this study revealed that the <i>ScCaM</i>
gene plays a negative regulatory role in both mannitol and NaCl stresses, and it possibly exerts protective mechanisms
common in both prokaryotes and eukaryotes under stress conditions.},
DOI = {10.32604/biocell.2022.022477}
}