TY - EJOU AU - LI, NINGYU AU - CHEN, XIAOFANG AU - GENG, SUXIA AU - LAI, PEILONG AU - HUANG, LISI AU - LI, MINMING AU - HUANG, XIN AU - DENG, CHENGXIN AU - WANG, YULIAN AU - WENG, JIANYU AU - DU, XIN TI - miR-103-3p regulates the differentiation of bone marrow mesenchymal stem cells in myelodysplastic syndrome T2 - BIOCELL PY - 2023 VL - 47 IS - 1 SN - 1667-5746 AB - The pathogenesis of myelodysplastic syndrome (MDS) may be related to the abnormal expression of microRNAs (miRNAs), which could influence the differentiation capacity of mesenchymal stem cells (MSCs) towards adipogenic and osteogenic lineages. In this study, exosomes from bone marrow plasma were successfully extracted and identified. Assessment of miR-103-3p expression in exosomes isolated from BM in 34 MDS patients and 10 controls revealed its 0.52-fold downregulation in patients with MDS compared with controls (NOR) and was downregulated 0.55-fold in MDS-MSCs compared with NOR-MSCs. Transfection of MDS-MSCs with the miR-103-3p mimic improved osteogenic differentiation and decreased adipogenic differentiation in vitro, while inhibition of miR-103-3p showed the opposite results in NOR-MSCs. Thus, the expression of miR-103-3p decreases in MDS BM plasma and MDS-MSCs, significantly impacting MDS-MSCs differentiation. The miR-103-3p mimics may boost MDS-MSCs osteogenic differentiation while weakening lipid differentiation, thereby providing possible target for the treatment of MDS pathogenesis. KW - Myelodysplastic syndrome KW - Mesenchymal stem cells KW - miR-103-3p KW - Osteogenic differentiation KW - Adipogenic differentiation DO - 10.32604/biocell.2022.022021