@Article{biocell.2023.022937,
AUTHOR = {ZHUO YANG, WEN QIN, DI CHEN, JUNSHENG HUO, JINGBO WANG, LIYUAN WANG, QIN ZHUO, JIYONG YIN},
TITLE = {<i>In vitro</i> study of emodin-induced nephrotoxicity in human renal glomerular endothelial cells on a microfluidic chip},
JOURNAL = {BIOCELL},
VOLUME = {47},
YEAR = {2023},
NUMBER = {1},
PAGES = {125--131},
URL = {http://www.techscience.com/biocell/v47n1/49928},
ISSN = {1667-5746},
ABSTRACT = {Emodin is an effective component of rhubarb with positive pharmacological effects on human health. However, it is
also toxic to different cells or tissues to varying degrees. The effects of emodin on glomerular endothelial cells (GECs) remain to
be tested, and the documented works were always performed <i>in vitro</i> and hardly reflect the real physiological situation. To
study the effects of emodin on GECs in a biomimetic environment, we utilized a microfluidic chip to assess the
physiological reaction of human renal glomerular endothelial cells to various concentrations of emodin in this work. The
results showed that emodin caused cytotoxicity, impaired glomerular filtration barrier integrity to macromolecules, and
increased barrier permeability in a dose-dependent manner. With the increase in emodin concentration, the concentration
of the pro-inflammatory cytokine tumor necrosis factor-α, interleukin (IL)-6, transforming growth factor-β1, and
monocyte chemoattractant protein (MCP-1) increased while the production of inflammatory cytokine IL-6 first increased
and then decreased with the increase in emodin concentration. Our findings shed new light on emodin-induced
nephrotoxicity and provide insights for the application of microfluidic chip devices to reveal drug-cell interactions.},
DOI = {10.32604/biocell.2023.022937}
}