@Article{biocell.2023.025593,
AUTHOR = {TIANZHU LI, YU ZHANG, TONG ZHANG, YANNAN LI, HUI XUE, JINGLONG CAO, WENSHUANG HOU, YINGHUA LUO, CHENGHAO JIN,},
TITLE = {Schisandrin B exerts anticancer effects on human gastric cancer cells through ROS-mediated MAPK, STAT3, and NF-κB pathways},
JOURNAL = {BIOCELL},
VOLUME = {47},
YEAR = {2023},
NUMBER = {1},
PAGES = {195--204},
URL = {http://www.techscience.com/biocell/v47n1/49933},
ISSN = {1667-5746},
ABSTRACT = {Schisandrin B (Sch B) is a monomer with anti-cancer and anti-inflammatory effects, which are isolated from the
plant <i>Schisandra chinensis</i> (Turcz) Baillon. We investigated the anti-gastric cancer (GC) effects of Sch B and its
underlying molecular mechanisms. The Cell Counting Kit-8 assay was used to determine the effects of Sch B on the
viability of GC and normal cell lines. Hoechst/propidium iodide staining and flow cytometry were used to assess the
apoptosis induction of Sch B. Western blotting was used to evaluate the effects of Sch B on downstream apoptotic
proteins. The DCFH-DA fluorescent probe was used to assess the regulatory effects of Sch B on reactive oxygen
species (ROS) levels and related signaling pathways in GC cells. The results showed that Sch B could regulate the
phosphorylation level of mitogen-activated protein kinase (MAPK) by upregulating ROS accumulation in gastric
cancer cells, and then reduce the expression of nuclear factor kappa B (NF-κB) and phosphorylated transcription
3 (p-STAT3). In addition, Sch B downregulated the cell cycle proteins cyclin-dependent kinase 2/4/6 and cyclin D1/E,
and arrested cells in the G0/G1 phase. Moreover, it also inhibited cell migration, which was reversed with Nacetylcysteine pretreatment. In summary, Sch B has killing effects on GC cells by upregulating the production of
intracellular ROS and regulating the MAPK/STAT3/NF-κB signaling pathway, leading to the migration arrest and
apoptosis of GC cells.},
DOI = {10.32604/biocell.2023.025593}
}