@Article{biocell.2023.029860,
AUTHOR = {JIAZHUANG LI, WEI ZHANG, SHOUBAO GAO, LI SUN, QINGYANG TAI, YING LIU},
TITLE = {Long non-coding RNA-ATB induces trastuzumab resistance and aggravates the progression of gastric cancer by repressing miR- 200c via ZNF217 elevation},
JOURNAL = {BIOCELL},
VOLUME = {47},
YEAR = {2023},
NUMBER = {10},
PAGES = {2313--2320},
URL = {http://www.techscience.com/biocell/v47n10/54525},
ISSN = {1667-5746},
ABSTRACT = {<b>Background:</b> Trastuzumab resistance accounts for chemotherapy failure in gastric cancer patients in clinical
practice. The significance of long non-coding RNAs (lncRNAs) in the maintenance of drug resistance in gastric
cancer has been already underlined. <b>Method:</b> This study aimed to identify the specific role of lncRNA-ATB in gastric
cancer progression and trastuzumab resistance. The downstream miRs of lncRNA-ATB and target genes of miRs were
predicted by bioinformatics analysis and verified using dual luciferase reporter assay. Loss- and gain-function assays
were performed to explore the roles of lncRNA-ATB, miR-200c, and zinc-finger protein 217 (ZNF217) in the cell
functions and trastuzumab resistance of a trastuzumab-resistant gastric cancer cell line (NCI-N87-TR). <b>Result:</b>
LncRNA-ATB was upregulated, while miR-200c was downregulated. Depletion of lncRNA-ATB or miR-200c
elevation led to a decrease in malignant properties of NCI-N87-TR cells. LncRNA-ATB could negatively target miR-
200c, which in turn inversely targeted and reduced the expression of ZNF217. Silencing of ZNF217 could inhibit cell
viability and migration. <b>Conclusion:</b> lncRNA-ATB promoted the progression and trastuzumab resistance of gastric
cancer by repressing miR-200c via ZNF217 upregulation.},
DOI = {10.32604/biocell.2023.029860}
}