@Article{biocell.2023.030032,
AUTHOR = {HAOSHENG ZHENG, RUIJUN LIN, WEIJIE CAI, YUZHEN ZHENG, XINGPING YANG, ZUI LIU, FEI QIN, YONGJIE CAI, XIANYU QIN, HONGYING LIAO},
TITLE = {Analysis of functional hub genes indicates DLGAP5 is linked to lung adenocarcinoma prognosis},
JOURNAL = {BIOCELL},
VOLUME = {47},
YEAR = {2023},
NUMBER = {11},
PAGES = {2453--2469},
URL = {http://www.techscience.com/biocell/v47n11/54710},
ISSN = {1667-5746},
ABSTRACT = { <b>Introduction:</b> The difficulty in treating lung adenocarcinoma (LUAD) is caused by a shortage of knowledge about the biological mechanisms and a lack of treatment choices. <b>Objectives:</b> The aim of this study was to identify a valuable molecular target for the treatment of LUAD. <b>Methods:</b> Using multiple databases, we screened for hub genes in LUAD using Cytoscape and explored the expression and prognosis of DLG associated protein 5 (DLGAP5) in LUAD. We investigated the genetic variation, functional enrichment, and epigenetic activity of DLGAP5. Furthermore, we evaluated the relationship between the tumor microenvironment (TME) and DLGAP5. <b>Results:</b> Our study identified 10 hub genes in LUAD: CDC45, KIAA0101, DLGAP5, CDT1, NCAPG, CCNB1, CDCA5, CDC20, KIF11, and AURKA. We discovered that DLGAP5 was overexpressed and associated with poor prognosis in LUAD. DLGAP5 exhibited an overall genetic variation frequency of 2%, and its DNA promoter was hypomethylated in LUAD (<i>p</i> < 0.05). The expression of DLGAP5 in LUAD showed a positive correlation with the majority of N6-methyladenosine (m6A)-methylation genes. Additionally, DLGAP5 was primarily associated with the cell cycle in LUAD. Notably, there was a significant favorable association between DLGAP5 and CD274, CTLA4, HAVCR2, and LAG3 in LUAD. <b>Conclusion:</b> DLGAP5 may be a therapeutic target for LUAD, as it affects cancer cells proliferation and development through the regulation of cell-cycle checkpoints and modulation of immune cell infiltration and immune checkpoints in the TME.},
DOI = {10.32604/biocell.2023.030032}
}