@Article{biocell.2023.029687,
AUTHOR = {SHUHUI WU, PING XU, XIANGBIN XU, SONG-BAI LIU},
TITLE = {Research progress and prospects of nucleic acid isothermal amplification technology},
JOURNAL = {BIOCELL},
VOLUME = {47},
YEAR = {2023},
NUMBER = {11},
PAGES = {2385--2395},
URL = {http://www.techscience.com/biocell/v47n11/54725},
ISSN = {1667-5746},
ABSTRACT = {Nucleic acid (DNA and RNA) detection and quantification methods play vital roles in molecular biology. With
the development of molecular biology, isothermal amplification of DNA/RNA, as a new molecular biology technology,
can be amplified under isothermal condition, it has the advantages of high sensitivity, high specificity, and high
efficiency, and has been applied in various fields of biotechnology, including disease diagnosis, pathogen detection,
food hygiene and safety detection and so on. This paper introduces the progress of isothermal amplification
technology, including rolling circle amplification (RCA), nucleic acid sequence-dependent amplification (NASBA),
strand displacement amplification (SDA), loop-mediated isothermal amplification (LAMP), helicase-dependent
amplification (HDA), recombinase polymerase amplification (RPA), cross-priming amplification (CPA), and its
principle, advantages and disadvantages, and application development are briefly summarized.},
DOI = {10.32604/biocell.2023.029687}
}