TY  - EJOU
AU  - WU, JINSONG 
AU  - ZHI, ZHENG 
AU  - XU, WENZHONG 
AU  - LI, DIANCGENG 
AU  - LI, QIUBO 
AU  - HAN, YAN 
AU  - HE, JIANMING 
AU  - LIANG, XI 

TI  - LIM1863 is useful to explore collective cancer cell migration, and the group of heterogeneous cells undergoing collective migration behaves like a supracellular unit
T2  - BIOCELL

PY  - 2023
VL  - 47
IS  - 12
SN  - 1667-5746

AB  - <b>Introduction:</b> Collective cancer cell migration (CCCM) and epithelial-to-mesenchymal transition (EMT) play key roles in metastasis. This study reports that the colorectal carcinoma cell line LIM1863 is useful for the study of CCCM and EMT. <b>Methods:</b> Hematoxylin and eosin staining, scanning electron microscopy, transmission electron microscopy, and western blot analysis were performed. <b>Results:</b> LIM1863 automatically grew as spheroids in suspension and had important typical epithelial properties, including several layers of cells arranged around a central lumen, apical-basal polarity, and types of cell-cell junctions. Treatment with a combination of both TGF beta 1 and TNF alpha induced definite and distinct EMT, a spheroid changing phenotype to form a monolayer high-confluent patch without lumen, without polarity. Spontaneous CCCM occurred in spheroids. Flat EMT cells adhered to the base of a dish, exhibited persistent movement as a cluster of cells, and then shed, resulting in a cluster. All cells from one cluster undergoing CCCM died. Otherwise, all cells undergoing EMT disappeared and almost all cells located in the cell reservoir survived and proliferated. <b>Conclusion:</b> LIM1863 is an excellent cell line to study CCCM and EMT. The group of heterogeneous cells undergoing CCCM behaves like a supracellular unit.
KW  - Colorectal cancer; Epithelial-to-mesenchymal transition; Collective cell migration; Supracellular migration; Metastasis

DO  - 10.32604/biocell.2023.043494