@Article{biocell.2023.044314,
AUTHOR = {WEILING ZHANG, YONG LI, CAN ZHANG, QING HAN, YU ZHANG, AIQIN HE, WEIPEI ZHU},
TITLE = {Knockdown <i>Annexin A8</i> inhibits the proliferation and invasion of cervical cancer cells},
JOURNAL = {BIOCELL},
VOLUME = {47},
YEAR = {2023},
NUMBER = {12},
PAGES = {2697--2708},
URL = {http://www.techscience.com/biocell/v47n12/54984},
ISSN = {1667-5746},
ABSTRACT = {<b>Background:</b> This study aimed to explore the expression, function, and molecular mechanism of <i>ANXA8</i>, the gene for annexin 8, in cervical cancer. <b>Methods:</b> The gene expression of the <i>ANX</i> family members in cervical cancer tissues was classified via The Cancer Genome Atlas (TCGA) database. The expression of <i>ANXA8</i> in paracancerous tissues, cervical cancer tissues, and cell lines was identified by fluorescence quantitative polymerase chain reaction (PCR) and immunohistochemistry. The effects of <i>ANXA8</i> knockdown on the cellular growth and cell invasion of cervical cancer were examined by MTT, clone-formation assay, scratch test, and Transwell assay. The effect of <i>ANXA8</i> knockdown on the proliferative potency of cervical cancer cells was assessed through an <i>in vivo</i> nude-mouse tumor-formation test. The gene expression levels of Ki-67 and <i>ANXA8</i> in tumor-bearing tissues were measured through Immunohistochemical analysis. <b>Results:</b> TCGA data analysis and fluorescence quantitative PCR exhibited significantly increased expression of <i>ANXA8</i> in cervical cancer tissues. Further examination exhibited that <i>ANXA8</i> was expressed exceedingly in cervical cancer tissue, and it was associated with lymph node metastasis, FIGO stage, degree of differentiation, and infiltration depth of cancer patients. Cell-function assays revealed that knocking down <i>ANXA8</i> may substantially suppress the propagation, colony formation, invasion, and migration of cervical cancer cells. <i>In vivo</i> experiments demonstrated that <i>ANXA8</i> knockdown had a substantial inhibitory effect on the propagation of cervical cancer cells in nude mice and inhibited the expression of Ki-67. <b>Conclusion:</b> <i>ANXA8</i> is specifically and significantly upregulated within cervical cancer tissues. The knockdown of this gene showed remarkable outcomes, as evidenced by the substantial inhibition of cervical cancer cell proliferation in <i>in vitro</i> and <i>in vivo</i> experimentations. Therefore, <i>ANXA8</i> is a potential target or a marker that can serve as a therapeutic or diagnostic tool for cervical cancer.},
DOI = {10.32604/biocell.2023.044314}
}