@Article{biocell.2023.025377,
AUTHOR = {JING LUO, JIANPING HE, YONG LUO, CHENG YI},
TITLE = {<i>ENST00000535926</i> is an unfavorable prognosis-related and tumor-promoting transcript of the <i>CHPF</i> gene in luminal A and B breast cancer},
JOURNAL = {BIOCELL},
VOLUME = {47},
YEAR = {2023},
NUMBER = {2},
PAGES = {309--318},
URL = {http://www.techscience.com/biocell/v47n2/50474},
ISSN = {1667-5746},
ABSTRACT = {Chondroitin sulfate synthase 2 (<i>CHPF</i>) is characterized as an oncogenic and poor prognosis-related gene in breast cancer. However, this gene has alternative splicing products encoding proteins of different lengths. Breast cancer is a group of heterogeneous tumors with distinct clinical and genomic characteristics. In this study, we explored the expression profile and prognostic value of the two transcripts of <i>CHPF</i> using data from The Cancer Genome Atlas (TCGA)-BRCA. The functional regulation of the two transcripts was also studied in MCF-7 and BT-474 cells. Among the two transcripts of <i>CHPF</i>, <i>ENST00000535926</i> expression was significantly upregulated in the tumor samples and was the dominant isoform. <i>ENST00000535926</i>, but not <i>ENST00000243776</i> upregulation, was associated with significantly worse progression-free survival (PFS) and disease-specific survival (DSS) in luminal A/B cases. However, no significant association was observed in PFS or DSS in other Prediction Analysis of Microarray 50 (PAM50) subgroups. CHPF isoform 2 protein (encoded by <i>ENST00000535926</i>) significantly elevated the expression of <i>P3H1</i> and <i>RCN3</i> at the mRNA and protein levels in MCF-7 and BT-474 cells. The effect of <i>ENST00000535926</i> was significantly stronger than <i>ENST00000243776</i> in promoting tumor cell colony formation. The expression of <i>P3H1</i> and <i>RCN3</i> was negatively correlated with CD8+ T cell infiltration but was positively correlated with cancer-associated fibroblast infiltration in luminal A/B tumors. In summary, this study revealed that <i>ENST00000535926</i> is an unfavorable prognosis-related and tumor-promoting transcript of the <i>CHPF</i> gene in luminal A/B breast cancer.},
DOI = {10.32604/biocell.2023.025377}
}