@Article{biocell.2023.026182,
AUTHOR = {QIAOJIN JIA, YUEJUN FU},
TITLE = {The microRNA pathway activation in insect cell model upon <i>Autographa californica</i> multiple nucleopolyhedrovirus infection},
JOURNAL = {BIOCELL},
VOLUME = {47},
YEAR = {2023},
NUMBER = {3},
PAGES = {627--645},
URL = {http://www.techscience.com/biocell/v47n3/51094},
ISSN = {1667-5746},
ABSTRACT = {<b>Background:</b> The immune system of insects exerts fundamentally different antiviral mechanisms than
mammals. MicroRNAs (miRNAs) play vital roles in developing insect antiviral immunity. MiRNAs expression
profiles of insects changed significantly during baculovirus infection. <b>Methods:</b> Differential expression profiles of
miRNAs in <i>Spodoptera frugiperda</i> were monitored by next-generation sequencing (NGS) and RT-qPCR during
<i>Autographa californica</i> multiple nucleopolyhedrovirus (AcMNPV) infection. The transcription levels of genes were
detected by RT-qPCR. The 50% tissue culture infective dose (TCID50) endpoint dilution assay was used to determine
the proliferation of progeny virus. <b>Results:</b> NGS revealed that 49 miRNAs were differentially expressed in Sf9 cells,
and 10 of them were significantly up- or down-regulated. Though RT-qPCR analysis, we observed the similar trends
for the expression patterns of significantly differentially expressed miRNAs from NGS. Moreover, the transcription
levels of core genes, <i>Exportin5</i>, <i>Dicer1</i>, and <i>Argonaute1</i>, in miRNA biogenesis pathways were significantly increased
after AcMNPV infection. For five selected miRNAs, miR-34-5p could regulate the proliferation of baculovirus
progeny virus and energy metabolism. <b>Conclusion: </b>The miRNAs biogenesis pathway in Sf9 cells plays an important
role and may be stimulated to resist AcMNPV infection. This work provides evidence for the molecular mechanism
of baculovirus-insect interaction and offers novel ideas and directions for green pest control technology.},
DOI = {10.32604/biocell.2023.026182}
}