@Article{biocell.2023.026445,
AUTHOR = {HAOTONG SUN, HEYING WANG, XIN LI, YANJIE HAO, JUN LING, HUAN WANG, FEIMIAO WANG, FANG XU},
TITLE = {Increased <i>MAD2L2</i> expression predicts poor clinical outcome in Colon Adenocarcinoma},
JOURNAL = {BIOCELL},
VOLUME = {47},
YEAR = {2023},
NUMBER = {3},
PAGES = {607--618},
URL = {http://www.techscience.com/biocell/v47n3/51096},
ISSN = {1667-5746},
ABSTRACT = {<b>Background:</b> Colon adenocarcinoma (COAD) is the second leading cause of cancer death worldwide thus,
identification of COAD biomarkers is critical. Mitotic Arrest Deficient 2 Like 2 (<i>MAD2L2</i>) is a key factor in
mammalian DNA damage repair and is highly expressed in many malignant tumors. This is a comprehensive study
of <i>MAD2L2</i> expression, its diagnostic value, prognostic analysis, potential biological function, and impact on the
immune system of patients with COAD. <b>Methods:</b> Gene expression, clinical relevance, prognostic analysis, diagnostic
value, GO/KEGG cluster analysis, data obtained from TCGA, and bioinformatics statistical analysis were performed
using the R package. Immune responses to <i>MAD2L2</i> expression in COAD were analyzed using TIMER. The
expression of <i>MAD2L2</i> in HCT116 cells induced by the inflammatory factor TNF-α was detected using Western blot.
<b>Results:</b> Our results underscore the clinical diagnostic value and potential biological significance of <i>MAD2L2</i> in
patients with COAD. A high level of <i>MAD2L2</i> expression has been found in COAD and correlated with tumor status
and colon polyps. ROC curve analysis showed that <i>MAD2L2</i> expression has high diagnostic value in COAD. Analysis
of immune infiltration results showed that <i>MAD2L2</i> expression was positively correlated with neutrophil levels. The
western blot results demonstrated that <i>MAD2L2</i> was dose-dependently present with TNF-α. GO/KEGG revealed that
<i>MAD2L2</i> overexpressed and coexpressed genes were mostly involved in biological functions, including hypoxia
response, response to reduced oxygen levels, mitochondrial translation elongation, and other processes. <b>Conclusion:</b>
<i>MAD2L2</i> as a new COAD biomarker contributes to our understanding of how alterations in gene expression and the
immunological environment contribute to the development of colon cancer. Following further investigation, <i>MAD2L2</i>
may prove to be a viable target factor for clinical diagnosis and therapy of COAD.},
DOI = {10.32604/biocell.2023.026445}
}