@Article{biocell.2023.026122,
AUTHOR = {YIFAN XU, DONGMEI CHENG, LEI HU, XIN DONG, LIYING LV, CHEN ZHANG, JIAN ZHOU},
TITLE = {Single-cell sequencing analysis reveals the molecular mechanism of promotion of SCAP proliferation upon AZD2858 treatment},
JOURNAL = {BIOCELL},
VOLUME = {47},
YEAR = {2023},
NUMBER = {4},
PAGES = {825--836},
URL = {http://www.techscience.com/biocell/v47n4/51785},
ISSN = {1667-5746},
ABSTRACT = {The Wnt/β-catenin signaling pathway is the main target of tooth regeneration regulation. Treatment of cells with AZD2858 stimulates the Wnt/β-catenin signaling pathway, yet the function of this pathway in tooth regeneration remains unclear. Here, we found that AZD2858 promotes the accumulation of β-catenin in the nuclei of stem cells from the apical papilla (SCAPs) and enhances cell proliferation. Single-cell sequencing was performed on SCAPs treated with AZD2858. Eight clusters were identified, namely SCAPs-CNTNAP2, SCAPs-DTL, SCAPs-MYH11, SCAPs-MKI67, SCAPs-CXCL8, SCAPs-TPM2, SCAPs-IFIT2 and SCAPs-NEK10. The pseudo-time trajectory analysis showed that AZD2858 enhanced the evolution of SCAPs from SCAPs-TMP2 clusters to SCAPs-MYH11, SCAPs-CNTNAPs and SCAPs-NEK10 clusters via up-regulation of <i>PRKCA</i>, <i>SMURF2</i>, <i>MAGI2</i>, <i>RBMS3</i>, <i>EXT1</i>, <i>CAMK2D</i>, <i>PLCB4</i>, and <i>PLCB1</i>. These results demonstrate that AZD2858 enhances the proliferation of SCAPs-TPM2 cluster by activating the non-canonical Wnt/β-catenin signaling pathway.},
DOI = {10.32604/biocell.2023.026122}
}