@Article{biocell.2023.023874,
AUTHOR = {SHU ZHAO, SHIXIN YE},
TITLE = {Light-controlled phosphorylation in the TrkA-Y785 site by photosensitive UAAs activates the MAPK/ERK signaling pathway},
JOURNAL = {BIOCELL},
VOLUME = {47},
YEAR = {2023},
NUMBER = {6},
PAGES = {1377--1388},
URL = {http://www.techscience.com/biocell/v47n6/52792},
ISSN = {1667-5746},
ABSTRACT = {<b>Background:</b> This paper aims to establish a light-controlled phosphorylation detection method at the Y785 site
of tropomyosin receptor kinase A (TrkA) receptor in mammalian cells by using genetic code expansion technology and
detecting the effects of optical activation of this site on the downstream MAPK/ERK pathway. The study is based on the
current situation that the regulatory mechanism of TrkA phosphorylation has not been fully elucidated. <b>Methods:</b> Two
photosensitive unnatural amino acids, p-azido-L-phenylalanine (AzF) and photo-caged tyrosine (ONB) were introduced
into the TrkA-Y785 site by genetic code expansion technology and site-directed mutagenesis. Western blotting and laser
confocal imaging were conducted to analyze the effects of this site on activating the MAPK/ERK pathway and nerve cell
differentiation before and after photostimulation. <b>Results:</b> Our results supplemented the light-controlled results of the
TrkA-Y785 site based on our previous research and verified that Y785 also makes important contributions in
regulating the MAPK/ERK pathway. <b>Conclusion:</b> This study demonstrated the significant contributions of the TrkAY785 site in regulating the ERK pathway by precisely controlling the phosphorylation state of a single tyrosine site.},
DOI = {10.32604/biocell.2023.023874}
}