TY  - EJOU
AU  - ZHAO, SHU 
AU  - YE, SHIXIN 

TI  - Light-controlled phosphorylation in the TrkA-Y785 site by photosensitive UAAs activates the MAPK/ERK signaling pathway
T2  - BIOCELL

PY  - 2023
VL  - 47
IS  - 6
SN  - 1667-5746

AB  - <b>Background:</b> This paper aims to establish a light-controlled phosphorylation detection method at the Y785 site
of tropomyosin receptor kinase A (TrkA) receptor in mammalian cells by using genetic code expansion technology and
detecting the effects of optical activation of this site on the downstream MAPK/ERK pathway. The study is based on the
current situation that the regulatory mechanism of TrkA phosphorylation has not been fully elucidated. <b>Methods:</b> Two
photosensitive unnatural amino acids, p-azido-L-phenylalanine (AzF) and photo-caged tyrosine (ONB) were introduced
into the TrkA-Y785 site by genetic code expansion technology and site-directed mutagenesis. Western blotting and laser
confocal imaging were conducted to analyze the effects of this site on activating the MAPK/ERK pathway and nerve cell
differentiation before and after photostimulation. <b>Results:</b> Our results supplemented the light-controlled results of the
TrkA-Y785 site based on our previous research and verified that Y785 also makes important contributions in
regulating the MAPK/ERK pathway. <b>Conclusion:</b> This study demonstrated the significant contributions of the TrkAY785 site in regulating the ERK pathway by precisely controlling the phosphorylation state of a single tyrosine site.
KW  - Tropomyosin receptor kinase A
KW  -  Genetic code expansion
KW  -  Y785
KW  -  P-azido-L-phenylalanine
KW  -  Photo-caged tyrosine

DO  - 10.32604/biocell.2023.023874