TY  - EJOU
AU  - CHEN, LI 
AU  - LI, FANGFANG 
AU  - CAO, SHOUYAN 
AU  - LI, XIA 
AU  - ZHOU, CHAO 
AU  - HAN, SAI 
AU  - ZHANG, YOUZHONG 

TI  - RASAL2 acts as a tumor suppressor in cervical cancer cells
T2  - BIOCELL

PY  - 2023
VL  - 47
IS  - 7
SN  - 1667-5746

AB  - <b>Background: </b>This study was designed to investigate the roles of RASAL2 in cervical cancer (CC). <b>Methods:</b>
Fifty-four CC tissues and 33 adjacent tissues were obtained from CC patients admitted to our hospital between March
2012 and June 2014. Real-time polymerase chain reaction and western blotting were performed to analyze the
expression of RASAL2 mRNA and protein in these tissues, CC cell lines, and normal cervical cells. Over-expression
and silencing of RASAL2 were induced after transfection, and the migration, invasion, and proliferation of the CC cell
lines were examined. <b>Results:</b> RASAL2 mRNA and protein expressions were significantly down-regulated in CC
tissues and cell lines than in adjacent tissues and normal cervical cells, respectively. While low RASAL2 expression
correlated with advanced stage and metastasis of CC, its over-expression significantly inhibited proliferation and
metastasis of CC cells and induced apoptosis. Under <i>in vitro</i> conditions, silencing of RASAL2 expression could
significantly increase the proliferation, invasion, and migration of CC cells. <b>Conclusion:</b> RASAL2 functioned as a tumor suppressor in CC, and was down-regulated in CC tissue samples and cell lines.
tumor suppressor in CC, and was down-regulated in CC tissue samples and cell lines.
KW  - RASAL2
KW  -  Cervical cancer
KW  -  Knockdown
KW  -  Silencing
KW  -  Tumor suppressor

DO  - 10.32604/biocell.2023.027308