TY  - EJOU
AU  - JIANG, LIJUN 
AU  - JI, WENMIN 
AU  - GONG, YAJIE 
AU  - LI, JIAJUN 
AU  - LIU, JINCHUN 

TI  - MiR-520f-3p inhibits epithelial-mesenchymal transition of colorectal cancer cells by targeting Yes-associated protein 1
T2  - BIOCELL

PY  - 2023
VL  - 47
IS  - 8
SN  - 1667-5746

AB  - <b>Background:</b> Colorectal cancer (CRC) is one of the most common malignancies. Early diagnosis is the key to
effective treatment of CRC. Since microRNAs (miRNAs) can be used as biomarkers of CRC, the objective of this work was
to examine the effect of miR-520f-3p, which targets YAP1 (Yes-associated protein 1), on the ability of CRC cells to
proliferate, invade, migrate, and undergo epithelial-mesenchymal transition (EMT). <b>Methods:</b> A miR-520f-3p mimic
was used to overexpress miR-520f-3p in HT29 cells. To establish the tumor-bearing mouse model, transfected HT29
cells were subcutaneously implanted into BALB/c-nu nude mice, and YAP1 and miR-520f-3p levels were determined
using qRT‒PCR. The viability, invasion ability, and migration ability of cells were evaluated by CCK-8, Transwell, and
wound healing assays. Apoptosis was detected by flow cytometry and TUNEL assays. The regulatory link between
miR-520f-3p and the YAP1 gene was examined by dual-luciferase reporter assay. Tumor tissues with positive Ki-67
expression were identified by immunohistochemistry. Vimentin, E-cadherin, and YAP1 expression were evaluated by
western blotting. <b>Results:</b> MiR-520f-3p overexpression could inhibit proliferation, invasion, migration, and EMT and
induce apoptosis in HT29 cells. YAP1 was found as a target of miR-520f-3p. The inhibitory effects of miR-520f-3p on
proliferation, invasion, migration, and EMT may be reversed by overexpressing YAP1. In tumor-bearing mice, miR-
520f-3p overexpression reduced the Ki-67 level, increased apoptosis, and prevented tumor development and spread.
<b>Conclusion:</b> By targeting YAP1, miR-520f-3p may be capable of suppressing CRC cell proliferation, invasion,
migration, and EMT, providing a novel therapeutic target for the disease.
KW  - Colorectal cancer
KW  -  Epithelial interstitial transformation
KW  -  MiR-520f-3p
KW  -  YAP1

DO  - 10.32604/biocell.2023.029516