@Article{biocell.2023.024591,
AUTHOR = {WEI LU, KAN GUO, DIANMEI XI, ZHAOXIA XIA},
TITLE = {Exosomal miR-30a-5p targets NLRP3 to suppress podocyte pyroptosis in diabetic nephropathy},
JOURNAL = {BIOCELL},
VOLUME = {47},
YEAR = {2023},
NUMBER = {9},
PAGES = {1995--2008},
URL = {http://www.techscience.com/biocell/v47n9/54285},
ISSN = {1667-5746},
ABSTRACT = {<b>Background:</b> Mesenchymal stem cell (MSC)-derived exosomes are closely related to pyroptosis in diabetic
nephropathy (DN). This study aimed to explore the protective effect of exosomal miR-30a-5p on podocyte pyroptosis
in DN. <b>Methods:</b> Streptozotocin was used to establish the mouse model of DN. Human bone marrow MSC-derived
exosomes were extracted and identified via transmission electron microscopy, nanoparticle tracking analysis, and
western blotting. MiR-30a-5p mimics and non-control (NC) mimics were transfected into MSCs and podocytes, and
exosomes were isolated from the MSCs. High glucose (HG)-induced podocyte model was established to determine the
effect of exosomal miR-30a-5p on pyroptosis and inflammation in vitro. <b>Results:</b> MiR-30a-5p was expressed at low
levels in DN models, while NLR family pyrin domain containing 3 (NLRP3), caspase-1, gasdermin-N (GSDMD-N),
and pro-inflammatory factors (tumor necrosis factor-alpha, interleukin (IL)-1beta, and IL-18) were augmented. <i>In
vitro</i>, miR-30a-5p expression in the HG-damaged podocytes was down-regulated, while NLRP3 was up-regulated.
Interestingly, miR-30a-5p overexpression diminished HG-induced podocyte injury, as proven by increased activity
and decreased pyroptosis of podocytes. Concurrently, the up-regulation of miR-30a-5p could inhibit the expression of
pro-inflammatory factors, caspase-1, GSDMD-N, and NLRP3 in HG-induced podocytes. MSC-derived exosomal miR-
30a-5p treatment of HG-damaged cells has similar effects to miR-30a-5p mimics treatment. Overexpression of NLRP3
reversed the effect of miR-30a-5p mimics on HG-induced podocytes. <b>Conclusion: </b>This research confirmed that
exosomal miR-30a-5p regulates pyroptosis via mediating NLRP3 in DN.},
DOI = {10.32604/biocell.2023.024591}
}