@Article{biocell.2023.029581,
AUTHOR = {XUEJIN YANG, YUANYUAN ZHOU, XINYI PENG, XIAOHONG FU, JIANQING MA, JIANFENG LIU, DANDAN CAO},
TITLE = {Comparative transcriptome analysis provides insights into the molecular mechanism of the anti-nematode role of <i>Arachis hypogaea</i> (Fabales: Fabaceae) against <i>Meloidogyne incognita</i> (Tylenchida: Heteroderidae)},
JOURNAL = {BIOCELL},
VOLUME = {47},
YEAR = {2023},
NUMBER = {9},
PAGES = {2101--2113},
URL = {http://www.techscience.com/biocell/v47n9/54287},
ISSN = {1667-5746},
ABSTRACT = {<b>Background:</b> Plant root-knot nematode (RKN) disease is a serious threat to agricultural production across the
world. <i>Meloidogyne incognita</i> is the most prominent pathogen to the vegetables and cash crops cultivated. <i>Arachis
hypogaea</i> can effectively inhibit <i>M. incognita</i>, but the underlying defense mechanism is still unclear. <b>Methods:</b> In our
study, the chemotaxis and infestation of the second-stage juveniles (J2s) of <i>M. incognita</i> to <i>A. hypogaea</i> root tips were
observed by the Pluronic F-127 system and stained with sodium hypochlorite acid fuchsin, respectively. The
transcriptome data of <i>A. hypogaea </i>roots with non-infected or infected by J2s were analyzed. <b>Results:</b> The J2s could
approach and infect inside of <i>A. hypogaea</i> root tips, and the chemotactic migration rate and infestation rate were
20.72% and 22.50%, respectively. Differential gene expression and pathway enrichment analyses revealed ubiquinone
and other terpenoid-quinone biosynthesis pathway, plant hormone signal transduction pathway, and phenylpropanoid
biosynthesis pathway in <i>A. hypogaea</i> roots responded to the infestation of <i>M. incognita</i>. Furthermore, the <i>AhHPT</i>
gene, encoding homogentisate phytyltransferase, was considered to be an ideal candidate gene due to its higher
expression based on the transcriptome data and quantitative real-time PCR analysis. <b>Conclusion:</b> Therefore, the key
gene <i>AhHPT</i> might be involved in the <i>A. hypogaea</i> against <i>M. incognita</i>. These findings lay a foundation for revealing
the molecular mechanism of <i>A. hypogaea</i> resistance to <i>M. incognita</i> and also provide a prerequisite for further gene
function verification, aiming at RKN-resistant molecular breeding.},
DOI = {10.32604/biocell.2023.029581}
}