@Article{biocell.2023.030364,
AUTHOR = {MINGHONG WEI, CHENG XIAO, JIAN WU, LIHONG QIN, HONGLIANG LIU, YANG CAO, YUMIN ZHAO},
TITLE = {<i>MED4</i> gene positively affects preadipocyte differentiation in Chinese red steppe cattle},
JOURNAL = {BIOCELL},
VOLUME = {47},
YEAR = {2023},
NUMBER = {9},
PAGES = {2115--2123},
URL = {http://www.techscience.com/biocell/v47n9/54296},
ISSN = {1667-5746},
ABSTRACT = { <b>Background:</b> The regulatory genes related to lipid metabolism affect the intramuscular fat (IMF) content and improve meat quality traits. Mediator Complex Subunit 4 (MED4), a vitamin D receptor protein, affects vitamin D, livestock growth, carcass traits, and triglyceride deposition. However, the physiological function of the <i>MED4</i> gene on bovine adipocyte differentiation remains unknown. <b>Methods:</b> This study explored the function of the <i>MED4</i> gene in preadipocyte differentiation in Chinese Red Steppe cattle. The overexpression plasmid and the interference sequences of the <i>MED4</i> gene were constructed to detect the effects of the <i>MED4</i> gene on adipogenesis and biomakers using quantitative polymerase chain reaction and western blotting. <b>Results:</b> The <i>MED4</i> gene had significantly high expression during preadipocyte differentiation (<i>p</i> < 0.05). Overexpression of the <i>MED4</i> gene increased the expression of the <i>PPARγ</i> gene, a preadipocyte differentiation biomarker, improved cellular lipid droplets and triglycerides accumulation, and positively accelerated adipocyte maturation (<i>p</i> < 0.05). Interference of the <i>MED4</i> gene can negatively regulate preadipocyte differentiation. <b>Conclusion:</b> This study showed that the <i>MED4</i> gene may affect the preadipocyte differentiation and adipogenesis in Chinese Red Steppe cattle by regulating the <i>PPARγ</i> gene.},
DOI = {10.32604/biocell.2023.030364}
}