@Article{biocell.2024.058496,
AUTHOR = {ELMIRA YAKUPOVA, VALENTINA BABENKO, ALEXEY BOCHARNIKOV, KSENIYA FEDULOVA, DENIS SILACHEV, EGOR PLOTNIKOV},
TITLE = {Changes in bioenergetics and neuroprotective properties of mesenchymal stromal cells after LPS treatment},
JOURNAL = {BIOCELL},
VOLUME = {48},
YEAR = {2024},
NUMBER = {12},
PAGES = {1827--1834},
URL = {http://www.techscience.com/biocell/v48n12/59153},
ISSN = {1667-5746},
ABSTRACT = { <b>Background:</b> The active use of stem and progenitor cells in the therapy of various diseases requires the development of approaches for targeted modification of their properties. One such approach is the induction of a pro- or anti-inflammatory phenotype.  <b>Methods:</b> In this study, we investigated the effect of a pro-inflammatory environment <i>in vitro</i> on multipotent mesenchymal stromal cells (MSC) by incubation with lipopolysaccharide (LPS). iCELLigence real-time cell analysis system was used for monitoring cell culture growth. Cell energy metabolism was assessed using the Seahorse XFp Analyzer. For the rat stroke experiment, we used a photoinduced thrombosis (PT) model; after 24 h of surgery, vehicle or MSC or LPS-treated MSC was injected i.v. With magnetic resonance imaging (MRI) we evaluated the volume of ischemic brain injury. For the effect of MSC on neurological deficit after PT we used three methods: limb placement test, cylinder test, and beam-walking test.  <b>Results:</b> LPS exposure led to a significant increase in cell growth rate and to changes in their energy metabolism: glycolytic activity increased significantly in the MSC, and non-glycolytic acidification also increased, while basic respiratory parameters were maintained. With MRI we didn’t reveal changes in the volume of brain damage between all rat groups. Neurological deficit was less only with using untreated MSC injection.  <b>Conclusion:</b> Using LPS-treated MSC in the therapy of ischemic stroke in rats, we did not observe an increase in the neuroprotective properties of the cells, but instead noted some decrease in their therapeutic efficacy. We attribute these changes to the formation of a pro-inflammatory phenotype in MSC.},
DOI = {10.32604/biocell.2024.058496}
}