@Article{biocell.2024.047260,
AUTHOR = {LIJUAN ZHANG, QINGYIN MENG, LI ZHUANG, QUAN GONG, XIANDA HUANG, XUEQIN LI, SHIJUAN LI, GUOQIN WANG, XICAI WANG},
TITLE = {miR-30a-5p/PHTF2 axis regulates the tumorigenesis and metastasis of lung adenocarcinoma},
JOURNAL = {BIOCELL},
VOLUME = {48},
YEAR = {2024},
NUMBER = {4},
PAGES = {581--590},
URL = {http://www.techscience.com/biocell/v48n4/56129},
ISSN = {1667-5746},
ABSTRACT = { <b>Background:</b> Lung adenocarcinoma is a very pervasive histological form of lung cancers, and inhibiting metastasis is crucial for effective treatment. In this investigation, we explored the functional interaction of miR-30a-5p and the putative transcription factor 2 of the homeodomain (PHTF2) in dictating the aggressiveness and metastasis of lung adenocarcinoma. <b>Method:</b> We collected clinical samples to evaluate the expression patterns of miR-30a-5p and PHTF2 in lung adenocarcinoma along with normal tissues. Cellular experiments including cell count kit (CCK)-8 growth assay, apoptosis analysis, migration and invasion examinations were performed to assess the aggressiveness of lung adenocarcinoma cells. Furthermore, we examined tumorigenesis and metastasis in a nude mouse model. <b>Results:</b> MiR-30a-5p exhibited downregulation pattern in lung adenocarcinoma samples. Transfection of miR-30a-5p mimic in lung adenocarcinoma cells resulted in the suppression of malignant characteristics. Notably, the administration of miR-30a-5p mimic also curbed tumorigenesis and metastasis of lung adenocarcinoma cells in animal model. Moreover, PHTF2 was found to be a molecular target of miR-30a-5p. Upregulating PHTF2 counteracted the tumor-suppressive effect of the miR-30a-5p mimic. <b>Conclusion:</b> miR-30a-5p functions as a tumor-suppressive molecule while PHTF2 acts as an oncogenic factor in the development and metastasis of lung adenocarcinoma. Therefore, targeting miR-30a-5p and PHTF2 could be developed into a promising therapeutic approach for inhibiting metastasis in lung adenocarcinoma.},
DOI = {10.32604/biocell.2024.047260}
}