TY - EJOU
AU - ZHANG, LIJUAN
AU - MENG, QINGYIN
AU - ZHUANG, LI
AU - GONG, QUAN
AU - HUANG, XIANDA
AU - LI, XUEQIN
AU - LI, SHIJUAN
AU - WANG, GUOQIN
AU - WANG, XICAI
TI - miR-30a-5p/PHTF2 axis regulates the tumorigenesis and metastasis of lung adenocarcinoma
T2 - BIOCELL
PY - 2024
VL - 48
IS - 4
SN - 1667-5746
AB - Background: Lung adenocarcinoma is a very pervasive histological form of lung cancers, and inhibiting metastasis is crucial for effective treatment. In this investigation, we explored the functional interaction of miR-30a-5p and the putative transcription factor 2 of the homeodomain (PHTF2) in dictating the aggressiveness and metastasis of lung adenocarcinoma. Method: We collected clinical samples to evaluate the expression patterns of miR-30a-5p and PHTF2 in lung adenocarcinoma along with normal tissues. Cellular experiments including cell count kit (CCK)-8 growth assay, apoptosis analysis, migration and invasion examinations were performed to assess the aggressiveness of lung adenocarcinoma cells. Furthermore, we examined tumorigenesis and metastasis in a nude mouse model. Results: MiR-30a-5p exhibited downregulation pattern in lung adenocarcinoma samples. Transfection of miR-30a-5p mimic in lung adenocarcinoma cells resulted in the suppression of malignant characteristics. Notably, the administration of miR-30a-5p mimic also curbed tumorigenesis and metastasis of lung adenocarcinoma cells in animal model. Moreover, PHTF2 was found to be a molecular target of miR-30a-5p. Upregulating PHTF2 counteracted the tumor-suppressive effect of the miR-30a-5p mimic. Conclusion: miR-30a-5p functions as a tumor-suppressive molecule while PHTF2 acts as an oncogenic factor in the development and metastasis of lung adenocarcinoma. Therefore, targeting miR-30a-5p and PHTF2 could be developed into a promising therapeutic approach for inhibiting metastasis in lung adenocarcinoma.
KW - Lung cancer; Malignant phenotype; Tumor formation; Tumor suppressor; Oncogene
DO - 10.32604/biocell.2024.047260