@Article{biocell.2024.047404,
AUTHOR = {BIN LI, YUCHENG SHENG, XIAOYING XU, SHENGCUN WANG, HONGYAN SONG, JINGYUAN LI, HAONAN JI, QINGHUA WANG, XIAODI ZHOU, LONGJU QI},
TITLE = {Biological function of miRNA-145-5p in angiotensin II induced renal inflammation},
JOURNAL = {BIOCELL},
VOLUME = {48},
YEAR = {2024},
NUMBER = {4},
PAGES = {601--611},
URL = {http://www.techscience.com/biocell/v48n4/56130},
ISSN = {1667-5746},
ABSTRACT = { <b>Objective:</b> Chronic kidney disease (CKD) is a progressive disorder characterized by intricate structural and functional alterations in the kidneys, attributable to diverse causative factors. Notably, the therapeutic promise of miR-145-5p in addressing renal pathologies has been discerned. This investigation seeks to elucidate the functional role of miR-145-5p in injured kidneys by subjecting human glomerular mesangial cells (HGMCs) to stimulation with Angiotensin II (AngII). <b>Materials and Methods:</b> Cellular viability and the levels of inflammatory mediators were evaluated utilizing Cell Counting Kit-8 (CCK-8), quantitative real-time polymerase chain reaction (qRT-PCR), and western blot methodologies, both in the presence of AngII incubation and in scenarios of miR-145p overexpression and downregulation. Furthermore, the cell cycle dynamics were elucidated through Fluorescence-activated Cell Sorting (FACS) analysis. <b>Results:</b> AngII incubation induced an upregulation of miR-145-5p and inflammatory factors including Intercellular Adhesion Molecule 1 (ICAM-1), Interleukin 6 (IL-6), Interleukin 8 (IL-8), and Interleukin 1β (IL-1β). Additionally, it elevated the expression of Cyclin A2, Cyclin D1, and the G2/M cell cycle ratio. Conversely, inhibition of miR-145-5p heightened the levels of inflammatory factors and cell cycle regulators induced by AngII incubation. Reduced expression of miR-145-5p correlated with a downregulation of Interleukin 10 (IL-10) expression, concurrently promoting HGMC proliferation under AngII stimulation. Moreover, ectopic miR-145-5p expression demonstrated a reduction in inflammatory factors, cell cyclin regulators, G2/M cell cycle ratio, and overall proliferation. <b>Conclusion:</b> MiR-145-5p exhibited inhibitory effects on the inflammatory response and proliferation induced by Angiotensin II in HGMCs, showcasing its potential as a therapeutic avenue for the treatment of kidney injury.},
DOI = {10.32604/biocell.2024.047404}
}