TY  - EJOU
AU  - TIAN, KAIWEN 
AU  - CHEN, HANZHONG 
AU  - WANG, QIANQIAN 
AU  - JIANG, FENGLIAN 
AU  - FENG, CHUNXIANG 
AU  - LI, TENG 
AU  - PU, XIAOYONG 
AU  - TANG, YANLIN 
AU  - LIU, JIUMIN 

TI  - CARD11 serves as a therapeutic biomarker for the drug therapies of ccRCC
T2  - BIOCELL

PY  - 2024
VL  - 48
IS  - 5
SN  - 1667-5746

AB  -  <b>Background:</b> The incidence of clear cell renal cell carcinoma (ccRCC) is globally high; however, despite the introduction of innovative drug therapies, there remains a lack of effective biomarkers for evaluating treatment response. Recently, Caspase recruiting domain-containing protein 11 (CARD11) has garnered attention due to its significant association with tumor development and the immune system. <b>Methods:</b> The expression of CARD11 mRNA and protein in ccRCC were analyzed by public database and immunohistochemistry. The focus of this study is on the epigenomic modifications of CARD11, its expression of ccRCC immunophenotype, and its correlation with response to immunotherapy and targeted therapy. Furthermore, to investigate the mechanism of this molecule’s influence on different biological behaviors of cells, cell tests <i>in vitro</i> have been conducted to observe the impact of its expression level. <b>Results:</b> CARD11 expression was upregulated which may be mainly modified by body methylation and was correlated with poor prognosis in ccRCC. In the tumor microenvironment of ccRCC, CARD11 expression was positively correlated with increased T lymphocyte infiltration and increased expression of inhibitory immune checkpoints. Moreover, ccRCC patients with high CARD11 expression had a better response to immunotherapy and targeted therapy. The knockdown of CARD11 ultimately suppressed the proliferation, migration, and invasion capabilities of ccRCC cells while simultaneously enhancing tumor cell apoptosis. <b>Conclusion:</b> We identified CARD11 as a novel therapeutic biomarker for immunotherapy and targeted therapy in ccRCC.
KW  - Clear cell renal cell carcinoma; Tumor microenvironment; CARD11; Immune checkpoint inhibitor; Tyrosine kinase inhibitor

DO  - 10.32604/biocell.2024.048737