@Article{biocell.2024.049349,
AUTHOR = {JIAWEI YIN, YONGSHENG WANG, GUANGWEI WEI, MINGXIN WEN},
TITLE = {UBE2T mediates the stemness properties of breast cancer cells through the mTOR signaling pathway},
JOURNAL = {BIOCELL},
VOLUME = {48},
YEAR = {2024},
NUMBER = {6},
PAGES = {959--970},
URL = {http://www.techscience.com/biocell/v48n6/57028},
ISSN = {1667-5746},
ABSTRACT = { <b>Objectives:</b> This study aimed to reveal the role and possible mechanism of the ubiquitin-conjugating enzyme 2T (<i>UBE2T</i>) in the biological activities of breast cancer stem cells (BCSCs). <b>Methods:</b> The specific protein and gene expression were quantified by Western blotting and quantitative real-time polymerase chain reaction, the proportion of BCSCs was examined by flow cytometry, and the self-renewal and proliferation of BCSCs were verified by serial sphere formation and soft agar. <b>Results:</b> Increasing expression of UBE2T was drastically found in breast cancer than that in adjacent tissues. Furthermore, UBE2T overexpression significantly increased the proportion of BCSCs in breast cancer cells and promoted their self-renewal and proliferation. Silent UBE2T exhibited the opposite functions. UBE2T increased the levels of the mammalian target of rapamycin and the phosphorylated mammalian target of rapamycin. Mammalian target of rapamycin (<i>mTOR</i>) inhibitor rapamycin inhibited the function of <i>UBE2T</i> in BCSCs. <b>Conclusion:</b> <i>UBE2T</i> plays a role in BCSCs through mTOR pathway and may suggest a novel therapeutic strategy for breast cancer.},
DOI = {10.32604/biocell.2024.049349}
}