TY  - EJOU
AU  - ZHONG, GUIBIN 
AU  - WANG, SHURONG 
AU  - HE, YUJIN 
AU  - FENG, DAMING 
AU  - WEI, KE 
AU  - YANG, YANQIU 
AU  - CHEN, JIANWEI 
AU  - CHEN, JUNLING 

TI  - Circular RNA circ_0003609 ameliorates hypertrophied ligamentum flavum by regulating the miR-155/SIRT1 axis
T2  - BIOCELL

PY  - 2024
VL  - 48
IS  - 6
SN  - 1667-5746

AB  -  <b>Background:</b> Hypertrophy of the ligamentum flavum (HLF) is a common contributor to spinal stenosis which results in significant neurological impairments. Circular RNA (circRNA) circ_0003609 has been linked to HLF; however, the exact mechanism by which it causes this disease is unclear. <b>Methods:</b> Circ_0003609 expressions were regulated in HLF cells by overexpression vectors and RNA interference. Cell proliferation and fibrosis-related gene expression were checked by the Cell Counting Kit-8 (CCK-8) assay and western blotting. CircBank’s prediction of the association between miR-155 and circ_0003609 was supported by a dual-luciferase reporter experiment. The function of the miR-155/sirtuin 1 (SIRT1) axis in controlling HLF fibrosis was further examined. <b>Results:</b> Overexpression of circ_0003609 suppressed HLF cell propagation and fibrosis compared to its silencing. It was found that circ_0003609 served as the sponge for miR-155 and that the circ_0003609/miR-155 axis controlled the fibrosis of HLF cells. It was found that circ_0003609 acted as a sponge for miR-155, regulating the fibrosis of HLF cells. Further, miR-155 targets SIRT1, and the miR-155/SIRT1 axis promotes HLF cell fibrosis. <b>Conclusion:</b> Circ_0003609 ameliorates hypertrophied ligamentum flavum (LF) by modulating the miR-155/SIRT1 axis, indicating a potential treatment approach for HLF.
KW  - Circular RNA; Circ_0003609; Hypertrophy of ligamentum flavum; MiR-155; Sirtuin 1; Fibrosis

DO  - 10.32604/biocell.2024.050294