@Article{biocell.2024.050493,
AUTHOR = {SIYUAN LIU, YUAN ZHAO, YU YU, DOU YE, QIAN WANG, ZHAOYAN WANG, ZUO LUAN},
TITLE = {Mesenchymal stromal cells modulate unfolded protein response and preserve β-cell mass in type 1 diabetes},
JOURNAL = {BIOCELL},
VOLUME = {48},
YEAR = {2024},
NUMBER = {7},
PAGES = {1115--1126},
URL = {http://www.techscience.com/biocell/v48n7/57221},
ISSN = {1667-5746},
ABSTRACT = { <b>Introduction:</b> Transplantation of mesenchymal stromal cells (MSCs) is a promising therapy for type 1 diabetes (T1D). However, whether the infused MSCs affect the endoplasmic reticulum stress or subsequent unfolded protein response in β cells remains unclear. <b>Methods:</b> To investigate this, we induced early-onset T1D in non-obese diabetic mice using streptozotocin. Subsequently, T1D mice were randomly assigned to receive either MSCs or phosphate-buffered saline. We observed the <i>in vivo</i> homing of MSCs and assessed their effectiveness by analyzing blood glucose levels, body weight, histopathology, pancreatic protein expression, and serum levels of cytokines, proinsulin, and C-peptide. <b>Results:</b> Infused MSCs were found in the lungs, liver, spleen, and pancreas of T1D mice. They exhibited various effects, including reducing blood glucose levels, regulating immunity, inhibiting inflammation, increasing β-cell areas, and reducing the expression of key proteins in the unfolded protein response pathway. Fasting serum proinsulin and C-peptide levels were significantly higher in the MSCs treatment group than in the T1D model group. However, there was no significant difference in the biomarker of β-cell endoplasmic reticulum stress, the ratio of fasting serum proinsulin to C-peptide, between the two groups. <b>Conclusion:</b> Our findings reveal that MSCs infusion does not alleviate endoplasmic reticulum stress in β cells directly but modulates the unfolded protein response pathway to preserve β-cell mass and function in T1D mice.},
DOI = {10.32604/biocell.2024.050493}
}