@Article{biocell.2024.050519,
AUTHOR = {XULONG SUN, WENTAO DING, CHAO JIANG, ZHIAN FANG},
TITLE = {miR-557 suppresses hepatocellular carcinoma cell proliferation and migration via downregulating CBX4},
JOURNAL = {BIOCELL},
VOLUME = {48},
YEAR = {2024},
NUMBER = {7},
PAGES = {1071--1079},
URL = {http://www.techscience.com/biocell/v48n7/57224},
ISSN = {1667-5746},
ABSTRACT = { <b>Introduction:</b> Hepatocellular carcinoma (HCC), a prevalent malignancy, poses significant challenges with high tumor heterogeneity and poor prognosis. MicroRNAs (miRNAs) play a pivotal role in hepatocarcinogenesis. Although abnormalities in microRNA-557 (miR-557) expression have been implicated in various cancer types, its role in HCC remains unclear. Therefore, there is a need to explore the function of microRNA-557 in HCC. <b>Methods:</b> Candidate miRNAs were identified through screening in GSE108724 and GSE20077. Real-time PCR was employed to analyze the expression level of miR-557 in hepatoma cell lines and tissues. Cell viability and migration assays were applied to assess the impact of miR-557 on HCC cell lines. Furthermore, the miR-557 target was predicted through three algorithms (Targetscan, miRWalk, and miRanda), and this was confirmed through luciferase assay and Western blotting. <b>Results:</b> In this study, miR-557 was identified in two datasets and expressed at a low level in both hepatoma cell lines and tissues. Notably, high expression of miR-557 in HCC cells inhibited oncogenesis. Conversely, low expression of miR-557 enhanced tumor proliferation and migration. Polycomb chromobox 4 (CBX4) was identified as a direct target of miR-557. Silencing CBX4 influenced the functional impact of miR-557 on HCC cell migration. <b>Conclusion:</b> Taken together, our study contributed to elucidating the hepatoma molecular heterogeneity and provided novel insights into miR-557 role and its target CBX4 in HCC, suggesting its potential as a future effectively druggable target for HCC intervention.},
DOI = {10.32604/biocell.2024.050519}
}