TY  - EJOU
AU  - SUN, XULONG 
AU  - DING, WENTAO 
AU  - JIANG, CHAO 
AU  - FANG, ZHIAN 

TI  - miR-557 suppresses hepatocellular carcinoma cell proliferation and migration via downregulating CBX4
T2  - BIOCELL

PY  - 2024
VL  - 48
IS  - 7
SN  - 1667-5746

AB  -  <b>Introduction:</b> Hepatocellular carcinoma (HCC), a prevalent malignancy, poses significant challenges with high tumor heterogeneity and poor prognosis. MicroRNAs (miRNAs) play a pivotal role in hepatocarcinogenesis. Although abnormalities in microRNA-557 (miR-557) expression have been implicated in various cancer types, its role in HCC remains unclear. Therefore, there is a need to explore the function of microRNA-557 in HCC. <b>Methods:</b> Candidate miRNAs were identified through screening in GSE108724 and GSE20077. Real-time PCR was employed to analyze the expression level of miR-557 in hepatoma cell lines and tissues. Cell viability and migration assays were applied to assess the impact of miR-557 on HCC cell lines. Furthermore, the miR-557 target was predicted through three algorithms (Targetscan, miRWalk, and miRanda), and this was confirmed through luciferase assay and Western blotting. <b>Results:</b> In this study, miR-557 was identified in two datasets and expressed at a low level in both hepatoma cell lines and tissues. Notably, high expression of miR-557 in HCC cells inhibited oncogenesis. Conversely, low expression of miR-557 enhanced tumor proliferation and migration. Polycomb chromobox 4 (CBX4) was identified as a direct target of miR-557. Silencing CBX4 influenced the functional impact of miR-557 on HCC cell migration. <b>Conclusion:</b> Taken together, our study contributed to elucidating the hepatoma molecular heterogeneity and provided novel insights into miR-557 role and its target CBX4 in HCC, suggesting its potential as a future effectively druggable target for HCC intervention.
KW  - Hepatocellular carcinoma; miR-557; Chromobox 4; Hepatocarcinogenesis

DO  - 10.32604/biocell.2024.050519