TY  - EJOU
AU  - CHINTAGUNTA, ANJANI DEVI 
AU  - KUMAR, SAMUDRALA PRASHANT JEEVAN 
AU  - KUMAR, NUNE SATYA SAMPATH 

TI  - Metabolic engineering and genome editing strategies for enhanced lipid production in microalgae
T2  - BIOCELL

PY  - 2024
VL  - 48
IS  - 8
SN  - 1667-5746

AB  - Depleting global petroleum reserves and skyrocketing prices coupled with succinct supply have been a grave concern, which needs alternative sources to conventional fuels. Oleaginous microalgae have been explored for enhanced lipid production, leading towards biodiesel production. These microalgae have short life cycles, require less labor, and space, and are easy to scale up. Triacylglycerol, the primary source of lipids needed to produce biodiesel, is accumulated by most microalgae. The article focuses on different types of oleaginous microalgae, which can be used as a feedstock to produce biodiesel. Lipid biosynthesis in microalgae occurs through fatty acid synthesis and TAG synthesis approaches. In-depth discussions are held regarding other efficient methods for enhancing fatty acid and TAG synthesis, regulating TAG biosynthesis bypass methods, blocking competing pathways, multigene approach, and genome editing. The most potential targets for gene transformation are hypothesized to be a malic enzyme and diacylglycerol acyltransferase while lowering phosphoenolpyruvate carboxylase activity is reported to be advantageous for lipid synthesis.
KW  - Oleaginous microalgae; Biodiesel; TAG synthesis; Metabolic engineering; Genome editing

DO  - 10.32604/biocell.2024.050540