TY  - EJOU
AU  - WANG, JING 
AU  - XU, JUNFENG 
AU  - YANG, YINGRAN 
AU  - QIU, YOUZHENG 
AU  - ZHANG, SHANSHAN 
AU  - WANG, NING 

TI  - Interferon-gamma regulates the progression of neuroblastoma cells through interferon-regulatory factor-1
T2  - BIOCELL

PY  - 2024
VL  - 48
IS  - 9
SN  - 1667-5746

AB  -  <b>Objective:</b> This study aimed to elucidate the influence of IFN-gamma (IFN-γ) in neuroblastoma (NB) cells and reveal its potential underlying molecular mechanism. <b>Methods:</b> The Cell Counting Kit-8, Transwell apparatus, and flow cytometry were employed to assess cellular viability, migratory capacity, invasive potential, and apoptotic rates, respectively. RNA-seq combined with bioinformatics analysis revealed differentially expressed genes (DEGs) and their possible biological functions. Protein levels were determined by western blot analysis. <b>Results:</b> IFN-γ treatment resulted in diminished cell viability, mitigated migratory and invasive capabilities, and augmented apoptotic activity in the SK-N-BE (2) cell line, whereas it exhibited the opposite effect in SH-SY5Y cells. Furthermore, interferon regulatory factor 1 (IRF-1) was the common DEG in both IFN-γ-treated SK-N-BE (2) and SH-SY5Y cells. Additionally, we found that it was underexpressed in NB tissues. The depletion of IRF-1 promoted the progression of both SK-N-BE (2) and SH-SY5Y cells. Moreover, IRF-1 knockdown effectively counteracted the effects of IFN-γ on SK-N-BE (2) cells, while exacerbating them in SH-SY5Y cells. <b>Conclusion:</b> This study verified that IFN-γ exerted a distinct role in both N-Myc-and non-N-Myc-amplified NB cells, partially by mediating the expression of IRF-1, suggesting that it may serve as a potent agent for treating patients with NB.
KW  - Neuroblastoma; N-Myc-amplified; Non-N-Myc-amplified; IFN-γ; IRF-1

DO  - 10.32604/biocell.2024.051673