@Article{biocell.2024.056698,
AUTHOR = {LIUJIE CHEN, LILI DUAN, JIA LI, JUN CHEN, DUANFANG LIAO, NONGYUE HE, KAI LI, ZHENG HU},
TITLE = {Advances in CRISPR-based gene editing technology and its application in nucleic acid detection},
JOURNAL = {BIOCELL},
VOLUME = {49},
YEAR = {2025},
NUMBER = {1},
PAGES = {21--43},
URL = {http://www.techscience.com/biocell/v49n1/59352},
ISSN = {1667-5746},
ABSTRACT = {Nucleic acid analysis is a key technique that enables accurate detection of various microorganisms. Conventional nucleic acid testing typically requires access to specialized laboratories, equipment, and trained personnel, which hinders the widespread use of on-site testing for DNA and RNA targets. However, integrating gene editing technology with traditional nucleic acid detection methods, especially isothermal amplification technology, can help overcome the limitations associated with on-site testing. This combination can accomplish precise and swift detection of nucleic acid sequences, offering a robust tool for on-site detection. The Clustered Regularly Interspaced Short Palindromic Repeats/CRISPR-associated proteins (CRISPR/Cas) technology, which comprises the CRISPR system and Cas effector proteins, is a powerful tool that is advancing the field of nucleic acid detection. Specifically, Cas12, Cas13, and Cas14 proteins have emerged as straightforward, effective, precise, sensitive, and cost-effective methods for <i>in vitro</i> nucleic acid detection because of their “collateral cleavage” characteristics. When combined with the “collateral cleavage” ability of Cas protein and isothermal amplification, CRISPR/Cas systems have great potential to advance nucleic acid detection. This article summarizes the research progress of different CRISPR/Cas systems and their applications in nucleic acid detection and future perspectives.},
DOI = {10.32604/biocell.2024.056698}
}