@Article{biocell.2025.061585,
AUTHOR = {Lingling Zhang, Hualing Wang, Yawen Liu, Ling Li, Jianping Xiong},
TITLE = {ANLN Promotes Cervical Cancer Cell Proliferation, Migration and Invasion and Suppresses Apoptosis via the Wnt/β-Catenin Pathway},
JOURNAL = {BIOCELL},
VOLUME = {49},
YEAR = {2025},
NUMBER = {2},
PAGES = {253--267},
URL = {http://www.techscience.com/biocell/v49n2/59704},
ISSN = {1667-5746},
ABSTRACT = { <b>Objective:</b> Anillin (ANLN) is considered an oncogene in various cancers, but its effect on cervical cancer remains poorly understood. Hence, this study aimed to describe the action of ANLN on cervical cancer development and investigate the potential mechanism. <b>Methods:</b> Analysis of ANLN expression and its association with survival in carcinoma and endocervical adenocarcinoma (CESC) patients based on GEO and UALCAN databases. The tumor and adjacent normal tissues of 100 cervical cancer cases were harvested to detect the ANLN expression and explore its relationship with patient survival. Cell proliferation, apoptosis, migration, and invasion were measured by utilizing 5-ethynyl-2<sup>′</sup>-deoxyuridine (EdU) staining, Flow cytometry, and Transwell assay, respectively. ANLN and Wnt expression were analyzed by RT-qPCR and Western Blot. <b>Results:</b> ANLN was significantly elevated in tumor tissues, and cervical cancer cases with high ANLN expression exhibited poor survival and high dead proportion. Besides, ANLN induced cervical cancer cell proliferation, migration, and invasion and restrained cell apoptosis. In addition, ANLN promoted Wnt/β-catenin pathway activation. Furthermore, ANLN accelerated cell aggressive behaviors and suppressed cell apoptosis via activating the Wnt/β-catenin signaling in cervical cancer. <b>Conclusion:</b> ANLN was enhanced in cervical cancer tissues and related to poor prognosis. ANLN accelerated cervical cancer cell aggressive behaviors and suppressed cell apoptosis via activating the Wnt/β-catenin pathway.},
DOI = {10.32604/biocell.2025.061585}
}