TY  - EJOU
AU  - Jiang, Luyao 
AU  - Fu, Longsheng 
AU  - Xiong, Shaofeng 
AU  - Cao, Guosheng 
AU  - Mei, Yanqin 
AU  - Wu, Yaoqi 
AU  - Chen, Jin 
AU  - LV, Yanni 

TI  - ScRNA-seq and Experimental Analyses Unveil Lrg1 Regulating the Oxidative Phosphorylation Pathway to Affect Neutrophil Accumulation after Cerebral Ischemia-Reperfusion
T2  - BIOCELL

PY  - 2025
VL  - 49
IS  - 9
SN  - 1667-5746

AB  -  <b>Background:</b> After ischemic stroke, neutrophils hyperactivate, increasing in number and worsening inflammation, causing neural damage. Prior scRNA-seq showed Lrg1 modulates cells subsentence to cerebral ischemia-reperfusion injury, but its mechanism in regulating neutrophil accumulation/differentiation post-injury is unclear. <b>Methods:</b> <i>Lrg1</i> knockout impact on neutrophil accumulation was assessed via immunofluorescence and western blot. Three-dimensional reconstruction of immunofluorescent staining analyzed cell-cell interactions among neutrophils and microglia. scRNA-seq of WT and <i>Lrg1</i><sup><i>-/-</i></sup> mice from GSE245386 and GSE279462 was conducted. Each group conducted oxidative phosphorylation scoring via Gene Set Enrichment Analysis (GSEA), while Metascape was employed to perform GO and KEGG enrichment analyses for elucidating functional mechanisms. CellChat exhibited cell-cell communication. Furthermore, alterations in microglial phagocytic activity were evaluated by immunostaining for CD68, a well-established marker of phagolysosomal activity in phagocytic cells. Brain energy metabolism was evaluated via glutamate dehydrogenase activity and ATP levels with ELISA, and enzyme expression was analyzed by immunofluorescence and western blot. <b>Results:</b> <i>Lrg1</i> knockout decreased neutrophil accumulation and NET formation in mice. 3D immunofluorescence reconstruction confirmed neutrophil co-localization with endothelial cells/microglia. scRNA-seq revealed that the oxidative phosphorylation score was significantly higher in the MCAO/R+WT group compared to both the Sham-operated+WT and <i>Lrg1</i><sup><i>-/-</i></sup> groups. Notably, the oxidative phosphorylation score was further elevated in the MCAO/R+<i>Lrg1</i><sup><i>-/-</i></sup> group. Immunostaining showed that <i>Lrg1</i> knockout elevated CD68+ lysosome expression post-MCAO/R, with TMEM119 colocalizing with these lysosomes. MCAO/R raised CD68 expression in ischemic brains, an effect further intensified by <i>Lrg1</i> knockout. KEGG analysis linked differential genes to oxidative phosphorylation pathways. Validation in MCAO/R vs. sham groups revealed increased ROS production and reduced expression of complex enzymes I-V (NDUFB8, SDHB, UQCRC1, MTCO2, ATP5A1). Lrg1 intervention increased enzyme expression. Immunofluorescence and western blot in brain tissue showed similar patterns in microglia and enzymes I-V. <b>Conclusions:</b> <i>Lrg1</i> knockout significantly enhances microglial phagocytic activity towards neutrophils subsequent to cerebral ischemia-reperfusion injury, through its regulatory effect on the oxidative phosphorylation pathway. This finding accentuates Lrg1 as a highly potential therapeutic target for intervening in and modulating post-ischemic inflammatory responses.
KW  - Leucine-rich alpha-2-glycoprotein 1; neutrophil; microglia; scRNA-seq; cerebral ischemia-reperfusion

DO  - 10.32604/biocell.2025.068507