@Article{cmc.2020.013646,
AUTHOR = {Shaheena Khanum, Muhammad Adeel Ashraf, Asim Karim, Bilal Shoaib, Muhammad Adnan Khan, Rizwan Ali Naqvi, Kamran Siddique, Mohammed Alswaitti},
TITLE = {Gly-LysPred: Identification of Lysine Glycation Sites in Protein Using Position Relative Features and Statistical Moments via Chou’s 5 Step Rule},
JOURNAL = {Computers, Materials \& Continua},
VOLUME = {66},
YEAR = {2021},
NUMBER = {2},
PAGES = {2165--2181},
URL = {http://www.techscience.com/cmc/v66n2/40677},
ISSN = {1546-2226},
ABSTRACT = {Glycation is a non-enzymatic post-translational modification which assigns sugar molecule and residues to a peptide. It is a clinically important attribute to numerous age-related, metabolic, and chronic diseases such as diabetes, Alzheimer’s, renal failure, etc. Identification of a non-enzymatic reaction are quite challenging in research. Manual identification in labs is a very costly and time-consuming process. In this research, we developed an accurate, valid, and a robust model named as Gly-LysPred to differentiate the glycated sites from non-glycated sites. Comprehensive techniques using position relative features are used for feature extraction. An algorithm named as a random forest with some preprocessing techniques and feature engineering techniques was developed to train a computational model. Various types of testing techniques such as self-consistency testing, jackknife testing, and cross-validation testing are used to evaluate the model. The overall model’s accuracy was accomplished through self-consistency, jackknife, and cross-validation testing 100%, 99.92%, and 99.88% with MCC 1.00, 0.99, and 0.997 respectively. In this regard, a user-friendly webserver is also urbanized to accumulate the whole procedure. These features vectorization methods suggest that they can play a critical role in other web servers which are developed to classify lysine glycation.},
DOI = {10.32604/cmc.2020.013646}
}