TY  - EJOU
AU  - Khanum, Shaheena 
AU  - Ashraf, Muhammad Adeel 
AU  - Karim, Asim 
AU  - Shoaib, Bilal 
AU  - Khan, Muhammad Adnan 
AU  - Naqvi, Rizwan Ali 
AU  - Siddique, Kamran 
AU  - Alswaitti, Mohammed 

TI  - Gly-LysPred: Identification of Lysine Glycation Sites in Protein Using Position Relative Features and Statistical Moments via Chou’s 5 Step Rule
T2  - Computers, Materials \& Continua

PY  - 2021
VL  - 66
IS  - 2
SN  - 1546-2226

AB  - Glycation is a non-enzymatic post-translational modification which assigns sugar molecule and residues to a peptide. It is a clinically important attribute to numerous age-related, metabolic, and chronic diseases such as diabetes, Alzheimer’s, renal failure, etc. Identification of a non-enzymatic reaction are quite challenging in research. Manual identification in labs is a very costly and time-consuming process. In this research, we developed an accurate, valid, and a robust model named as Gly-LysPred to differentiate the glycated sites from non-glycated sites. Comprehensive techniques using position relative features are used for feature extraction. An algorithm named as a random forest with some preprocessing techniques and feature engineering techniques was developed to train a computational model. Various types of testing techniques such as self-consistency testing, jackknife testing, and cross-validation testing are used to evaluate the model. The overall model’s accuracy was accomplished through self-consistency, jackknife, and cross-validation testing 100%, 99.92%, and 99.88% with MCC 1.00, 0.99, and 0.997 respectively. In this regard, a user-friendly webserver is also urbanized to accumulate the whole procedure. These features vectorization methods suggest that they can play a critical role in other web servers which are developed to classify lysine glycation.
KW  - Gly-LysPred; PseAAC; post-translational modification; lysine glycation; Chou’s 5 step rule; position relative features

DO  - 10.32604/cmc.2020.013646