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Phyton-International Journal of Experimental Botany
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DOI: 10.32604/phyton.2024.050928

REVIEW

Ecosystem Services of Grazed Grasslands in the Flooding Pampa

Elizabeth J. Jacobo1,*,# and Adriana M. Rodríguez2,#

1Facultad de Agronomía, Área de Agroecología, Universidad de Buenos Aires, Buenos Aires, 1417, Argentina
2Facultad de Agronomía, Departamento de Producción Animal, Universidad de Buenos Aires, Buenos Aires, 1417, Argentina
*Corresponding Author: Elizabeth J. Jacobo. Email: ejacobo@agro.uba.ar
#Both authors contributed equally to this work and share the first authorship
Received: 22 February 2024; Accepted: 30 May 2024; Published: 27 June 2024

Abstract: The Flooding Pampa grasslands are the last remnant of the Rio de la Plata grasslands in Argentina. Anthropogenic interventions have led to severe degradation and, as a result, the ecosystem services provided by the grasslands are declining, in terms of provisioning, regulating, and supporting services. We synthesized the existing literature on the ecosystem goods and services provided by these grasslands under grazing in different conditions and conservation status. We found that plant and animal diversity and primary production are the most studied ecosystem services, while climate regulation, water supply, nutrient cycling, meat production and erosion control, in that order, are less studied. Cultural services are under-researched. Continuous grazing and glyphosate spraying are the main drivers of grassland degradation. Controlled grazing and conservative stocking rates have been shown to reverse degradation and demonstrate that livestock production is compatible with ecosystem conservation by maintaining regulating and provisioning services. As these management strategies are poorly integrated, improving their implementation will require important changes in farmers’ decisions and the development of policies that create the economic conditions for this to happen. Research is needed to understand the conditions that prevent the knowledge generated from being transferred to producers and translated into practices that would improve the provision of ecosystem services.

Keywords: Salado basin; sustainability; biodiversity; rangelands; meat production; adaptative multi paddock grazing; process technologies; agroecology

1  Introduction

The Río de la Plata Grasslands (RPG) region covers approximately 853,000 km2 and includes the extensive plains of Argentina, Uruguay, and southern Brazil that surround the estuary of the Río de la Plata and its major tributaries [1]. The Pampas region, covering 398,966 km2 in central-eastern part of Argentina (Fig. 1), is one of the largest temperate grassland regions globally and the most significant grassland ecosystem in the country [2]. It is a strategic area, accounting for 40% of the agricultural land in the RPG region [3] and is home to almost half of Argentina’s population [4].

Figure 1: Pampa region and its subregions. Insert: location of Pampa region in Argentina

Within the Pampa region, different subregions are recognised based on biophysical characteristics [3] (Fig. 1). The Rolling and Flat Inland Pampa subregions, with deep and fertile soils that are highly suitable for agriculture, have undergone intense land use change, resulting in the replacement of approximately 75% of natural grassland with annual crops [2,5]. The Flooding Pampa subregion (90,000 km2) has an average annual rainfall that varies from 1000 mm in the north to 850 mm in the south, evenly distributed throughout the year, and monthly temperatures that range from 6.8°C in July–August to 21.8°C in January [6]. This sub-region is characterised by shallow soil depth, soil salinity [7] and frequent floods and droughts [8], which severely limit agricultural production, thus preserving a large area of natural or semi-natural grasslands1.

The proportion of natural or semi-natural grasslands area varies from 53% to 89%, depending on the district [10], with an average of 68% [11]. These grasslands are the main source of fodder for livestock, as extensive livestock farming has been the main economic activity since Spanish colonisation to the present day.

Natural grassland ecosystems provide several goods and services to society [12], both directly through the provision of food, and indirectly through supporting and regulating services [13]. However, the provision of ecosystem goods and services is strongly dependent on the state and condition of grasslands, caused by anthropogenic interventions [14–16].

Therefore, the objectives of this review article are (i) to describe the history of use and its effects on the Flooding Pampa grasslands, (ii) to synthesise the existing literature on the ecosystem goods and services provided by the Flooding Pampa grasslands under grazing in different condition and conservation status caused by human interventions, (iii) to analyse and discuss the existing work to complete the state of knowledge and suggest new research directions. The achievement of these objectives will permit the organization and updating of the state of the art regarding the provision of goods and services in the Flooding Pampa grasslands.

2  Materials and Methods

We conducted a comprehensive literature search on 05 February 2024 in order to identify ecosystem goods or services provided by the Flooding Pampa grasslands. We first used the free search engines Google Scholar (https://www.google.com/, accessed on 09/04/2024) and Research Gate (https://www.researchgate.net/, accessed on 09/04/2024) to search across a wide range of academic publications. We searched by the terms “Flooding Pampa AND Grazing” and “Flooding Pampa Grassland AND Ecosystem Services”, and the same terms in Spanish “Pampa Deprimida Pastoreo”, and “Pastizales Pampa Deprimida Servicios Ecosistémicos” within the time range from 1980 to 2023. A total of 1140 results were obtained, from which we selected articles, reviews and thesis that explicitly addressed some function, process, or indicator of ecosystem services provided by the Flooding Pampa grasslands. From this selection, 145 results were obtained. We also conducted a second literature search in the scientific database Scopus, Science Direct, Springer and JSTOR using the same terms in English within the same period. From this search we selected 77 scientific articles, all of them were included in the first search.

We considered the spatial scale, so we referred globally to the Rio de la Plata grasslands at the regional scale, to the Flooding Pampa grasslands at the subregional scale, and specifically to the different vegetation communities that make up the landscape of the Flooding Pampa subregion. We have included some references to the grasslands of other subregions of the RPG where relevant.

The main conceptual models related to the ecology and management of grasslands were reviewed in order to discuss their consistency with the empirical results obtained in the grazed grasslands of the Flooding Pampa.

We preceded the results related to some ecosystem goods and services with a brief theoretical framework when it was necessary to improve their interpretation.

We have included several case studies to illustrate the best practices that have led to the successful implementation of conservation strategies.

3  Results and Discussion

3.1 Conceptual Models and Evolutionary History

In grassland ecology and management science, the multiple equilibrium, state-and-transition [17] or state-and-threshold model [18] and the generalized model of the effects of grazing on plant community structure and diversity [19] have been widely accepted as conceptual frameworks that were supported by empirical evidence. The generalized model predicted the response of plant diversity to grazing intensity, as a function of rainfall availability (subhumid or semi-arid climate) and evolutionary history of grazing (long or short) and assumed that rangelands in southern South America had a short evolutionary history of grazing [19]. A reworking and combination of these models to include a wider range of real situations concluded that the rangeland systems of southern South America could have a longer evolutionary history of grazing [20]. Several examples supported this conclusion, such as the case of Flooding Pampas grasslands, which did not respond to the prediction of the generalized model because changes in vegetation structure caused by grazing were reversible, as predicted for systems with a long history [20] (Fig. 2). Other studies have confirmed that South America has a long evolutionary history of herbivory by large grazers [21,22]. Although large herbivores became extinct in South America at the end of the Pleistocene and the beginning of the Holocene [23], the period of about 10,000 years without grazing by large herbivores until the introduction of domestic livestock is considered insufficient to substantially change the regional grassland species pool and the absence of large grazers may have been a major factor that increased fire frequency at the end of the Pleistocene in the Rio de la Plata grassland [1]. Thus, in humid ecosystems fire maintained an analogous selective pressure for traits also adapted to grazing, such as rapid regrowth after biomass loss [24].

Figure 2: Diversity as function of grazing intensity for a site with high productivity that have evolved under long history of grazing, such as Flooding Pampa grassland. Vertical arrow indicates the grazing intensity after which irreversible transitions can occur. Adapted from [20,25]

3.2 Grazing and Degradation History

The Spanish colonizers introduced domestic livestock to Argentina in 1549. A second arrival of cattle in the Pampas region between 1570 and 1580 increased the size of the herds [26]. The temperate climate and herbaceous vegetation of the Pampas allowed for a high reproductive rate of cattle, resulting in a population of 20 million head by 1850 [26,27]. Consequently, Argentina became the primary supplier of leather and meat to Western Europe in the second half of the 19th century. Between 1875 and 1930, the land used for agricultural crops increased from 100,000 to 10 million hectares [28]. Since then, agriculture has continued to expand, leading to the conversion of natural grassland to crops. From 2001 to 2018, this process was the primary cause of land use change in the Pampa region, resulting in a decline in ecosystem functions and services [29,30].

Since Spanish colonization cattle grazed freely on the native grasslands until they were confined when the new owners fenced their properties in the early 20th century. The structure and function of Flooding Pampa grasslands were altered by the uncontrolled or continuous grazing of the introduced livestock. Continuous grazing by large, introduced herbivores has increased the relative contribution of annual and forbs, mainly exotic and cool-season species of Eurasian origin and reduced the relative contribution of warm-season native perennial grasses that dominated the original vegetation [31,32]. Although these changes have increased diversity by adding non-native species to the grassland, they have significantly reduced aboveground net primary production [33]. As a result, after almost 100 years of continuous grazing, stocking rates, secondary productivity and economic returns declined towards the end of the last century (1995) from 0.77 to 0.49 animal units ha−1, from 105.2 to 66.4 kg live weight ha−1 and from 14 to −4.4 u$s ha−1 ,respectively [34]. A more recent reassessment conducted in 2017 also showed a similar tendency to decline in stocking rates (from 1 to 0.6 animal units ha−1), secondary productivity (from 182 to 84 kg live weight ha−1) and economic returns (from 130 to −10 u$s ha−1) under continuous grazing [35].

At the beginning of this century, the process of agriculture expansion and intensification in the Pampa region that initiated, led to a drastic change in land use. This change was made possible by the introduction of genetically modified soybeans tolerant to glyphosate [36] and the elimination of soil tillage (no tillage) [37]. As a result, pastures and annual fodder crops have been replaced by wheat–soybean relay cropping and maize crops. Due to the decrease in land allocated for forage production in the fertile areas of the Pampas, cattle were moved to the less fertile areas, such as the Flooding Pampa. This led to an increase in the stocking rate in this subregion, where native grasslands were the dominant land use and were partially replaced by annual forage crops or cultivated pastures [38]. In this subregion, the main change in land cover was from natural grasslands to semi-natural grasslands. This change was attributed to the intensification of livestock activity [5]. Additionally, an increase in stocking rate may exacerbate overgrazing of remnant native grasslands, resulting in negative impacts on aboveground net primary productivity, plant and animal diversity, and soil organic carbon and erosion [11].

To manage with the higher stocking rate, farmers in the Flooding Pampa have adopted a new technology that involves using glyphosate and nitrogen fertilisation to increase forage productivity during winter, when forage production is typically lower. Glyphosate is sprayed in late summer to eliminate vegetation composed mainly of C4 grasses and forbs, and to improve the germination and establishment of cool-season (C3) annual grasses. The main component of these grasses is Lolium multiflorum. Initially, this practice increased winter forage production and allowed for improvements in stocking rates and meat production [39]. However, recurrent application of glyphosate dramatically alters the structure and function of the vegetation community. It greatly reduces species richness and diversity, causes the local extinction of several native perennial species [40], reduces the annual amount of ANPP and changes its seasonal pattern, resulting in a dramatic reduction in spring and summer [41].

Due to more than a century of uncontrolled or continuous grazing in most of the area and more than twenty years of glyphosate application in the most productive communities, natural grasslands have severely degraded. As a result of this degradation process, medium-scale traditional farmers have a low meat production of about 87.5 kg PV ha−1 year−1 [42].

3.3 Controlled Grazing as a Tool to Reverse Degradation

In the late 1990s studies demonstrated the superiority of rotational or Adaptative Multi Paddock Grazing (AMPG) over continuous stocking in maintaining productivity and conserving and restoring native grasslands in North America [43–45], Australia [46] and the Flooding Pampa [47,48]. Furthermore, recent research has found a positive association between AMPG and the physical wellbeing of Canadian ranchers [49].

In the Flooding Pampa, the AMPG was referred to as Controlled Grazing (CG). CG involves dividing the land surface into homogeneous areas based on the type of plant community and applying disturbances, mainly grazing with high instantaneous stocking rates followed by a resting period of variable duration. The grazing intensity, timing and resting period duration are determined based on the plant community, season and objective pursued. This includes restoring the vigour of species with high forage value, controlling selectivity, modifying competitive relationships between functional groups, promoting germination and establishment, as well as flowering and fruiting of desired species [34].

Long-term evaluation at farm scale showed that CG increased the contribution of functional group species of high forage value and reduced the proportion of less valuable forage grasses and forbs [48]. Consequently, the grassland condition index, an indicator of grassland health and productivity, was significantly higher on cattle farms that applied CG (average 75%) than on those that applied continuous grazing (average 20%) [50]. In non-halophytic communities, CG anticipated the establishment and increased the biomass production of winter grasses with high nutritive value [47]. This reduces the winter forage deficit, which is the main limitation for cattle production [34]. In halophytic communities, CG increased vegetation cover, species richness, the contribution of cool-season grasses and forage quality [51].

The statement that these grasslands have a long evolutionary history of herbivory by large grazers and/or fire, which maintained a selective pressure towards traits that are also adapted to grazing (see Section 3.1) is supported by the reversion of the degradation of vegetation structure and function via CG.

3.4 Ecosystem Services Provided by Grasslands at Different Conservation Status

Natural grassland ecosystems provide various goods and services to society [12], both directly through the provision of food, and indirectly through supporting and regulation services [13]. However, the provision of ecosystem goods and services depends heavily on the state and condition of the grassland, which is influenced by anthropogenic interventions [14–16]. The subsequent subsections summarise the primary goods and services offered by the Flooding Pampa grasslands under different conservation statuses resulting from human interventions.

3.4.1 Supporting Ecosystem Services

Supporting services refers to the fundamental ecosystem processes that contribute to human wellbeing and maintain the functions necessary for provisioning, regulating, and cultural services [52].

Habitat Provision: Animal Diversity

The Pampa grasslands currently host an estimated 300 bird species, 65 mammal species, 49 reptile species and 35 amphibian species. Agricultural intensification has had a negative impact on animal diversity because it has homogenised the landscape. Birds and carnivores have been more severely affected than rodents and insects. The geographic range and/or abundance of many native species has been reduced, including those of carnivores, herbivores, and specialists, sometimes leading to regional extinctions. Meanwhile some native species have been unaffected or even benefited, and novel species have been introduced [53].

The Flooding Pampa has a greater richness and abundance of birds compared to other pampa sub-regions where agricultural activity dominates and grassland coverage is less extensive. This highlights that livestock activity provides the best scenario for bird conservation [54]. The greater species richness of the Flooding Pampa is due to the higher presence of habitat-specific bird species, which are specialists in grassland habitat. Generalist species, on the other hand, are adapted to a variety of open habitats [55]. The rapid conversion of natural grasslands into agricultural land has a significant impact on grassland birds, whose reproduction is strictly dependent on these environments. Several threatened grassland specialists in the Pampas, such as Rhea americana and Asthenes hudsoni, are exclusively found in livestock landscapes, and the long-term survival of these species will depend on the conservation of this landscape type [56].

The structure of the vegetation affects bird richness [57]. To promote a diverse range of bird species, it is desirable to have a mosaic of short and tall native grasslands with patches of tussocks. The heterogeneous vegetation structure benefits various functional groups, as different grassland types elicit different responses to grassland birds [58]. The presence of tussocks, consisting of tall grasses such as Andropogon lateralis and Cortadeira selloana, have been shown to increase bird species diversity. This strategy has even been suggested for protecting endangered species [59]. Conversely, habitats with short grasses are necessary for certain migratory shorebird species [60].

Livestock management practices are used to modify vegetation structure, which can affect the abundance and structure of bird assemblages in the Flooding Pampa. Continuous grazing in livestock farming with homogenises grasslands in space and time leading to the replacement of grassland birds that are highly dependent on tall grasses with others that select short grasses [61]. However, the use of glyphosate to promote L. multiflorum has been found to decrease the abundance of short grassland birds due to the low vegetation cover in the lowest layer (less than 10 cm) of the canopy [62]. A recent study compared Grassland Alliance farms managed by CG with neighbouring farms managed by continuous grazing and found that bird species diversity was higher on the former. CG has allowed the establishment of populations of specialist grassland bird species, some of which are threatened with extinction [63]. The greater spatial and temporal variation in grassland height under CG allows for the presence of species with different habitat requirements for nesting and foraging [64].

Agricultural expansion has caused severe habitat loss for mammals. Large carnivores have become locally extinct [64], while populations of medium-sized carnivores and wild ungulates have significantly declined [64,65]. Additionally, rodents and other small mammals are reducing diversity, favouring more common species at the expense of rare or habitat-specific species [53,66]. It is worth noting that grasslands serve as refuges for several native species. The Tandilia mountain system, a grassland relict of the Southern Pampa and a contiguous subregion to the Flooding Pampa, contains 44% of the mammal diversity of the Pampas ecoregion. This includes species that are endemic to the ecoregion, globally declined species, and threatened species [67]. The selection of mammal species appears to be influenced by vegetation structure, with a preference for more complex and diverse habitats associated with extensive livestock production [68,69]. He pampas deer (Ozotocerus bezoarticus) is an endangered species [70] with small and highly isolated populations. There are only 200 individuals protected within the Campos del Tuyú National Park, in the Flooding Pampa. Habitat selection is affected by the presence of cattle, as deer prefer areas without them. However, when cattle are removed from plots, deer occupy them, suggesting that consumption is facilitated [65]. Research has demonstrated that large herbivores can enhance the consumption of small herbivores by consuming significant amounts of forage. This promotes the regrowth of higher quality tissues and improves the quality of the forage supply remaining for smaller herbivores [71]. The floristic composition and forage quality of two communities used by pampas deer were improved by the disturbance regime characteristic of CG, demonstrating the potential of CG to successfully address both productive objectives and the conservation of threatened herbivores [72].

Several farm-scale case studies have shown that CG enhances the diversity of birds and the presence of Pampas deer in livestock farms in the Flooding Pampa [73]. Therefore, CG can be considered a successful strategy for promoting wild fauna biodiversity.

Habitat Provision: Plant Diversity

The RPG are home of thousands of vascular plants species. About 8% of the species are endemic, while slightly more than 10% are naturalised exotic species [74]. The Flooding Pampa contains a total of 430 species, of which 21%–25% are naturalised exotic species, mostly of European origin. The native flora is composed of 141 species of grasses, 172 species of forbs, which only 15% being annuals. The annual species dominated the exotic flora, accounting for 72% of the total. Among them, annual forbs were the most prevalent, making up 58% [75].

Most exotic plants were likely introduced over 100 years ago [76]. The success of their invasion can be explained by their phylogenetic affinity and similarity on life forms to the native flora [77]. It is probably that most invasive species move from their native habitat to a similar one [78]. Invasion patterns are the result of interactions between the invasiveness of the available species, which is related to large seed size, and the invasibility of the community, which is related to its structure and disturbance [79]. Livestock grazing is considered a relevant disturbance that promotes the invasion of exotic species in Flooding Pampa grasslands [80].

The vegetation of the Flooding Pampa is arranged in complex mosaic of herbaceous plant communities. The species composition of these communities varies according to local variations in topography and soil salinity/alkalinity. The main communities are (i) Mesophytic meadows, which develop on positive and convex terrains, highest areas over typic Argiudolls soils; (ii) Humid mesophytic meadows, which develop on flat areas at intermediate topographic position over Natraquoll soils; (iii) Humid prairies, which develop on extended lowlands, with groundwater near the surface over Argialbolls soils; and (iv) Halophytic steppes, which develop on small depressions in flat areas, or rings surrounding humid areas over Mollic Natraqualfs soils [80]. Plant richness is highest in well-drained habitats at elevated topographic positions and declines towards lowland communities, where stressful conditions would determine that only a reduced number of species are able to establish [75].

The introduction of annual and exotic forbs due to continuous grazing by domestic herbivores has led to an increase in plant richness in the Flooding Pampa grassland [31,32]. Grazing prevents the dominance of a few tussock grasses and allows a diverse group of subordinate species to coexist at small scales [33]. Livestock grazing has been found to promote the introduction of exotic plant species and the coexistence of native ones, thereby enhancing plant diversity at the community level [80]. Furthermore, cattle dung serves as a vector for the dispersal of seeds of exotic species [81]. Consequently, certain exotic species such as L. multiflorum and Lotus tenuis have become stable and significant components of this grassland [82,83]. In the Ventania mountain system, which is another grassland relict in the Southern Pampa subregion, the maximum richness of a remnant of upland grassland was recorded at medium grazing intensities. This was due to a high number of forb species and winter grasses [84]. Additionally, at the landscape scale, continuous grazing has reduced the original heterogeneity of the grassland, resulting in a decrease in floristic diversity between communities ([75,80]).

The application of CG had a significant effect on the contribution of different functional groups in the most extensive communities. In the humid mesophytic meadows, CG increased the cover of legumes and C3 native and non-native grasses, both annual and perennial, while decreasing the cover of C4 prostate grasses and dicot forbs. In the humid prairie CG increased the cover of legumes and tussock hygrophytic grasses, while decreasing the cover of dicot forbs [48]. The floristic diversity remained unchanged in these non-halophytic communities. This was due to the reduction of dicots forbs and prostate grasses being offset by the concomitant increment in two non-native species of high forage value: L. multiflorum, a C3 annual grass with a high growing rate, and Lotus tenuis, a spring-summer legume [48]. CG promotes species of high forage value, mostly native, while reducing species of low or no forage value, mostly exotic. It also increases the contribution of cool-season forage species, resulting in a concomitant increase in carrying capacity (see section primary productivity and meat production). Additionally, the separation of areas with homogeneous vegetation (different communities), which is characteristic of this grazing method [34], helps to maintain the spatial heterogeneity of the grassland.

A long-term farm-scale case study demonstrated that in the halophytic communities, CG also caused favourable changes in vegetation structure. It increased vegetation cover and the contribution of warm and cool season native grasses, both annual and perennial, of higher forage value. Additionally, it encouraged the appearance of several grasses adapted to fertile, well-drained, and non-saline soils. As a result of these structural changes, species richness and diversity increased in this community [51]. The changes have been attributed to positive feedback, with increased organic carbon storage in soil resulting from increased fresh plant residue returns. This has led to an improvement in the physical environment of the topsoil due to the process of aggregate stabilisation. Furthermore, these beneficial changes are responsible for increased infiltration, which favours an incipient process of salt leaching and pH decreases [85].

The repeated use of glyphosate significantly changes the structure of vegetation in humid mesophytic meadows. It reduces species richness and diversity, causes the local extinction of several native perennial species, increases the basal cover of exotic cool-season annual grasses, and reduces the basal cover of cool-season perennial grasses, warm-season tussock grasses, warm-season legumes, and the vegetation cover in summer [40]. Furthermore, the intervention resulted in a significant alteration of the seed bank. Specifically, the density of cool-season annual grasses increased while that of cool and warm-season perennial grasses, sedges, legumes, and dicot herbs decreased. As a result, there was a decrease in both richness and diversity, and an increase in dominance [86].

3.4.2 Provision Ecosystem Services

Net Primary Productivity (NPP)

Mean annual precipitation is widely recognized as the primary driver of aerial net primary production (ANPP) in grasslands and shrublands [87,88]. This factor alone accounts for over 75% of the ANPP variation at a regional scale [89–91]. A regional scale estimation of ANPP of the Rio de la Plata grasslands during six years using remote sensing ranged from 5200 to 7860 kg ha−1 year−1, depending on the spatial location (subregions) (Table 1). The temporal variation of ANPP, ranged from 11% to 65% of the mean value of the ANPP for each location [92].

However, the differences among vegetation communities in the Flooding Pampa, which respond to soil traits and topographic position, result in different total annual and spatial patterns of ANPP [93,94]. The total annual ANPP estimated by biomass harvest ranges from 1500–2000 kg DM ha−1 year−1 for the most restricted community, the halophyte steppe, to 7500 kg DM ha−1 year−1 for the richest community, the mesophytic meadow, which is largely replaced by crops [33,93,95,96]. Intermediate values of ANPP are recorded in the humid mesophytic meadows, the most prominent community, ranging from 5800 to 4500 kg DM ha−1 year−1, and in the humid prairies, which reaches 6600 kg DM ha−1 year−1 [93,95,96]. However, estimations via remote sensing in the humid mesophytic meadow ranged from 6400–7200 kg DM ha−1 year−1 [97], and in the humid prairies found values around 4100–4500 kg DM ha−1 year−1 with an interannual variation ranging from 3993 to 4845 kg DM ha−1 year−1 [98] (Table 1).

In well-studied communities, grazing and other agricultural practices such as fertilization or herbicide spraying cause structural changes to vegetation cover, floristic composition, and functional groups. These changes, in turn, affects the quantity and seasonal pattern of primary productivity, as well as other ecosystem processes and services.

Grazing management has been found to modify the annual ANPP and the seasonal pattern of the humid mesophytic meadow. The ANPP for one year was 7200 kg DM ha−1 year−1 under grazing exclusion, 5700 kg DM ha−1 year−1 after mowing the vegetation of the exclosure, and 2250 kg DM ha−1 year−1 under continuous heavy grazing [33]. Two farm-scale experiments have shown that CG either increases annual ANPP or changes the seasonal pattern by increasing winter ANPP. The first experiment compared continuous grazing and CG at the same stocking rate during two consecutive years, one humid and one dry. The results found that CG modified the seasonal pattern of ANPP [47]. Winter forage productivity was 1377 and 1135 kg DM ha−1 year−1 under CG and 610 and 731 kg DM ha−1 year−1 under continuous grazing for the first and second year respectively, which represented an increase of 55%–125%. Summer forage productivity was 2127 and 1088 kg DM ha−1 year−1 under CG and 3250 and 1846 kg DM ha−1 year−1 under continuous grazing for the first and second year respectively. This represents a decrease of 52%–70%. An effective method to increase winter forage production is CG, which involves an intensive grazing period in early autumn to remove almost all available warm season biomass, followed by a resting period to allow seedling development and establishment of C3 grasses. The major contribution to winter forage production was L. multiflorum, which earlier establishment is the result of promoting germination through the perception of high red/far red ratios generated by very intense defoliation [47]. Therefore, it is recognized that CG may mitigate the negative impact of continuous grazing on winter forage availability in these grasslands. In the second experiment ANPP significantly increased in a humid mesophytic meadow that produced 2600 kg DM ha−1 year−1 under continuous grazing. After three years of applying CG, ANPP increased to 4800 kg DM ha−1 year−1 [99]. Both experiments were case studies in which CG was implemented at whole livestock farms, and the results were monitored for several years. Therefore, CG can be considered a successful strategy for increasing winter forage production and enhancing the seasonal pattern of ANPP.

The ANPP of halophytic steppes varied depending on the grazing method employed. Traditional continuous grazing resulted in an ANPP range of 2100 [93] to 2969 kg DM ha−1 year−1 [96]. Heavy continuous grazing decreased the ANPP to 1564 kg DM ha−1 year−1 [100]. After a minimum of seven years of applying CG, the ANPP increased to 4700 ± 797 kg DM ha−1 year−1, while grazing exclusion for seven to seventeen years allowed for an ANPP of 6274 ± 978 kg DM ha−1 year−1 [100] (Table 1). This study-case conducted at farm-scale demonstrated that even in this marginal community with high soil limitations, CG and grazing exclusion increases primary production and triggers positive ecosystem processes that reverses continuous grazing deterioration [51,85].

A two-year experiment demonstrated that spraying glyphosate in the humid mesophytic meadows community in late summer resulted in a reduction of the annual ANPP by approximately 16%–25% (3350 and 2918 kg DM ha−1 year−1 for the first and second year, respectively) compared to the unsprayed community (4005 and 3871 kg DM ha−1 year−1 for the first and second year respectively) [41] (Table 1). Between June and September, ANPP increased by 100% to 500%. However, during spring, summer, and autumn ANPP was 22% to 90% lower than the unsprayed grassland. Glyphosate spraying caused a significant alteration in the seasonal pattern of ANPP. This change in the seasonal pattern resulted from the reduced contribution of warm-season grasses and legumes, which are severely impacted by glyphosate spraying in late summer, when they are actively growing. Therefore, the higher forage supply in winter was unable to compensate for the reduction during the rest of the year. This may explain the recent trend of increasing the area cultivated with summer forage crops in the last years in the Flooding Pampa [41].

Fertilization with phosphate alone or in combination with nitrogen increased ANPP during both the cool and the warm seasons in the humid mesophytic meadows. This effect was observed at both plot-scale without grazing [101] and at farm-scale under CG [102] (Table 1). CG involves an intensive grazing period in early autumn to provide better growing conditions to C3 grasses by removing almost all available warm season biomass [47,48]. The release of competition and the provision of phosphorus alone or with nitrogen resulted in the promotion of C3 annual grasses and a significant increase in legume contribution, particularly Lotus tenuis. The annual ANPP of the humid mesophytic meadows increased by 30% to 100% compared to the control treatment, depending on the dose of fertilizer and the interannual variation of precipitation [102]. This community was fertilized for four years with 10 gm−2 year−1 NPK + micronutrients. It was then compared between seasonal grazing (rest during winter and spring and grazing during summer and autumn) and the grazing exclusion. The results showed that fertilization did not affect ANPP under exclosure, while it increased the ANPP of the seasonal grazing treatment by up to 103% [103].

Meat and Wool Production

ANPP is the primary factor determining of livestock biomass, particularly cattle and sheep, in South America [104]. Therefore, secondary production, including meat, milk, and wool, is also reliant to ANPP. While the conversion of photosynthetically active solar energy into net secondary production is inefficient, it is the sole means of converting the energy contained in rangelands vegetation, which is not suitable for human consumption, into animal products that are highly nutritious for humans. Globally, rangeland ecosystems provide 75 trillion MJ of animal products annually, assuming a utilization efficiency of 20% and a conversion efficiency of 2% of the consumed biomass into animal products. This value could potentially increase with improved management strategies that enhance energy capture, utilization efficiency, or conversion efficiency of consumed biomass to animal products [105].

The estimated production of meat equivalent (meat + milk + wool) in the RPG is 78 kg live weight ha−1 year−1, with a spatial range from 92.5 to 35.2 kg live weight ha−1 year−1 [1]. These estimations are consistent with historical data on meat production in the Flooding Pampa subregion, which ranged from 60 to 80 kg live weight ha−1 year−1 [106,107]. In 2019, the cattle stock in the Flooding Pampa was 8727.092 animals, with 6.674.346 falling under the cow-calf operation categories (cows, heifers, calves, and bulls). This represents 16% of the country’s stock and 46% of the Buenos Aires province [108]. We estimated the total volume of meat equivalent production in the Flooding Pampa subregion applying the procedure proposed in [1] which yields a value of 4560.000 t of live weight year−1. Multiplying the number of weaned calves derived from backgrounding per year in the subregion yields a similar value of 460.000 t of live weight year−1. This volume represents 15% of the total slaughtered bovine in 2022 [109], which feeds 9 million of Argentinian people per year, based on a per capita beef meat consumption of 51 kg year−1 [109]. In 2022, the sheep livestock stock of Flooding Pampa was around 781,000 animals [108], which produced 2132 tons of greasy wool [110].

Meat production in the Flooding Pampa is highly dependent on the livestock production model, including livestock system, inputs level, and grazing method. The suitability of the environment, as indicated by the relative proportions of the different communities, determines the achievable meat production. The model characterised by the predominant use of natural grassland in good condition through CG (low input farms) significantly increases meat production compared to Continuous Grazing. This is a consequence of higher PPNA and/or higher zootechnical value of the grassland under CG [50,99]. In pure cow-calf farming systems with no agricultural environment (mesophytic meadows) and therefore with grassland covering the entire area, CG allows for the achievement of 100 kg live weight ha−1 year−1. In backgrounding farming systems where up to 25% of the agricultural environment is replaced by forage crops and the CG allows the achievement of 200 kg live weight ha−1 year−1. The conversion of a small area to pasture on suitable soils not only results in the provision of higher-quality forage but also allows for more effective management of the natural grassland, as it affords greater flexibility in determining rest periods. Full cycle farming systems, which have a larger area of pasture and forage crops and use feedlot finishing, while continuously grazing the remaining grassland, achieve a live weight of 360 kg ha−1 year−1. However, it has been found that increased meat production in systems that replace more than 25% of the grassland (high input farms) significantly increases fossil energy consumption and reduces energy use efficiency [50]. On a farm undergoing agroecological conversion, meat production increased from 91 to 182 kg. live weight ha−1 year−1 after 3 years of being implemented CG and sowing 15% of the area with forage crops, resulting in a significant increase in gross margin [99]. The CG model yields higher profit, income stability and gross margin compared to the continuous grazing model. The latter lacks economic viability, even under different input-output price ratio scenarios [35]. These models are based on real farming systems and supported by empirical data, making them valuable case studies to highlight the CG as a suitable strategy for enhancing several provision services such as primary productivity and meat production.

Genetic Resources

The Flooding Pampa grasslands provide native genetic resources, allowing the identification of variability in different plant species traits. These are sources of genes for breeding programmes and to restore degraded habitats.

Two grass species, Elymus scabrifolius and Stapfochloa berroi (formerly Chloris berroi), are being studied for their potential to tolerance to saline–alkaline conditions [111,112]. These grasses are native to South America and have high forage value. E. scabrifolius is a perennial cool-season grass while S. berroi is a perennial warm-season grass. They are rarely found under continuous grazing due to their highly preference by herbivores. S. berroi is a significant component of the halophytic steppes under CG or grazing exclosure [51]. Meanwhile, E. scabrifolius is found in the upland communities of the Samboromboy Bay under seasonal grazing exclusion [73].

Two other native cool-season grasses which belong to the mesophytic and humid mesophytic meadows, Bromus catharticus and Bromus auleticus, are highly productive and digestible, and have been extensively studied due to their widespread use as forage resources. Bromus catharticus is an annual/biennial native fodder grass. Its high genetic diversity has allowed for the development of breeding programmes to obtain diverse commercial cultivars [113]. Bromus auleticus is a perennial native grass with high genetic diversity and potential for use in breeding cultivars and restoring degraded grasslands [114]. Both species were promoted in the upland communities of the Samborombom Bay under seasonal grazing exclusion [73].

Paspalum dilatatum is a perennial warm-season C4 grass native to South America. It is a primary component of the humid mesophytic meadows and humid prairies communities of the Flooding Pampa grasslands, providing high-quantity forage in large quantities during the warm-season [41]. P. dilatatum is more tolerant to frosts than other C4 grasses and can adapt to various edaphic and environmental conditions. Additionally, it is resistant to defoliation and has excellent regrowth capacity. Several cultivars were developed through conventional breeding in Argentina [115–117] and Australia. Currently, efforts are focused on transgenic and genome editing approaches [118].

3.4.3 Regulating Ecosystem Services

Climate regulation

Research on climate regulation in the Flooding Pampa grassland region has focused on estimating the carbon footprint, modelling strategies to reduce greenhouse gas emissions, estimating the greenhouse gas balance, and measuring changes in soil SOC stocks due to management practices.

SOC stocks are considered an intermediate ecosystem service that contributes to climate regulation. Grasslands are a crucial ecosystem for carbon storage, complementing forests in the fight against climate change [119]. However, grasslands’ contributions to human and planetary well-being have been widely overlooked, and their preservation, conservation, and restoration are of utmost importance. The global success of carbon sequestration depends on the complete and effective restoration of grassland ecosystems [120]. The RPG are an important carbon sink, estimated to store 5% of Latin America’s total soil carbon, despite covering less than 3% of the subcontinent’s total area [121].

Management practices change soil stocks in the Flooding Pampa grasslands. Soil organic matter levels were significantly higher in healthy native tall tussock grasslands compared to sown pastures [122]. Additionally, the application of glyphosate to promote L. multiflorum resulted in a reduction of SOC by 1,058 t ha−1 year−1 [41]. In a review at global level, grazing has been shown to have a negative effect on soil organic carbon (SOC) compared to non-grazed situations [123]. However, in the Flooding Pampa grazing promotes carbon accumulation compared to non-grazed systems [124,125]. The method of grazing significantly affects SOC stocks. CG has been found to increase soil C stocks by 1.48 t ha−1 year−1 in the lowland alkaline sites occupied by the halophytic steppe community [100] and by 0.539 t ha−1 year−1 in the highland or intermediate sites, occupied by the humid mesophytic meadow community [41] when compared to continuous grazing.

Using whole-farm modelling, the carbon footprint of cow-calf systems in the Flooding Pampa was estimated to be between 19–22 t CO2-eq/t LW [126–128]. When estimating the carbon footprint of real farms values ranged from 12.66 to 16.89 t CO2-eq/t LW [126]. Mitigation strategies for Flooding Pampa have been proposed through production intensification. The modelling of GHG emissions from cow-calf farms revealed that systems that include backgrounding with grain supplementation emit less GHG per unit of product than pure cow-calf systems [128]. Additionally, incorporating 10% of the farm area into fescue pasture and advancing the first service of heifers (from 27 to 15 months) reduced GHG intensity emissions by up to 17% [126].

Two models of livestock systems were found in the Flooding Pampas based on empirical data obtained from several real livestock farming systems used as case studies. The estimation of GHG balance showed a significant difference between the two models. The model characterized by the predominant use of natural grassland through CG (grassland system) emitted 2273 kg CO2 eq·ha−1 year−1 and sequestered carbon at a rate of 1851 kg CO2 eq·ha−1 year−1. However, the model associated with a greater expanse of pasture and forage crops as well as a higher animal stocking rate (intensive system), emitted 4500 kg CO2 eq·ha−1 year−1 and reduced SOC at a rate of 601 kg CO2 eq·ha−1 year−1. The lower GHG emissions of the grassland system were attributed to the lower emissions of CH4 and N2O resulting from the lower stocking rate. The elevated carbon sequestration observed in the grassland system was attributed to an increase in carbon sequestration capacity of the grassland in good condition, resulting from the implementation of CG. Consequently, the GHG balance was approximately ten times more negative in the intensive system than in the CG grassland system, which was nearly neutral [129].

A single measurement experiment was conducted to compare N2O winter emissions in soils with and without grazing in different patches of grazed Flooding Pampa grasslands. The emissions of N-N2O from the urine patches were higher than those from faeces and control treatments, with average values of 26.13, 5.35 and 2.88 µg N-N2O/m2h, respectively. Additionally, the N-N2O emissions increased after rainfall [130].

Nutrient Cycling and Erosion Control

Soil nitrogen (N) content is influenced by both plant community and grazing treatment. In the lowland community, where high C content is likely due to the export of dissolved nutrients from upland to lowland sites, continuous grazing increased N content with respect to exclosure conditions [124]. The significantly lower amount of C and N measured in this community soil under exclosure reflected a major change in the pattern of element cycling, which resulted in large quantities of C and N flowing through the aboveground vegetation pathway in absence of domestic herbivores [124]. However, in the upland community continuous grazing either had no effect on the mineral N content of the soil [124] or decreased it [131]. The extractable phosphorus (P) content did not change under continuous grazing [127] or decreased with respect to the grazing exclusion [131].

Continuous grazing affects nutrient cycling by altering both the floristic composition of the vegetation and the soil conditions for decomposition. Forbs dominate continuously grazed grasslands and have higher litter quality than grasses that dominate ungrazed grasslands [132]. Grazed soil environments increase decomposition rates relative to those without grazing [133]. The application of glyphosate significantly decreased the total soil P content and had a tendency to reduce soil N content when compared to unsprayed grasslands. This is due to changes in vegetation function and structure, which may alter litter quantity and quality [41].

The application of CG significantly improved soil physical properties related to erosion control. In the upland community, topsoil had 16% lower bulk density and 54% lower surface bearing capacity compared to continuous grazing [134]. A long-term case study of a livestock farming system with a high proportion of alkaline lowland soils demonstrated that CG improved the physical properties of this community by reducing bulk density, structural instability, salinity and bearing capacity of the topsoil [85].

An experiment was conducted to estimate the sediment concentration of runoff in grasslands of different conditions in a catchment of Rolling Pampa subregion using simulated rainfall. The results showed that sediment concentration was 3.5 times higher in degraded grasslands than in healthy ones [135].

Water Regulation

Changes in land use, such as cropping or afforestation, have affected the regulating services provided by Flooding Pampa grasslands at the landscape scale. A catchment study that includes part of the Flooding Pampa found a strong direct relationship between the loss of grasslands to cropping and the reduction in in lentic water bodies [136]. Afforestation resulted in increased water uptake, which lowered groundwater levels by about 0.5 m compared to the surrounding grassland [137].

At a plot scale, the vegetation structure had an impact on the water dynamic of the grassland. The infiltration rate was significantly higher, and the bulk density was lower in the native tall tussock grasslands compared to the native short-grass grasslands of the Flooding Pampa. Furthermore, the simulation of water dynamics under drought conditions indicated that transpiration from native tall tussock was higher and evaporation was lower than that from native short grass. This reflects the high productivity of native tall tussock grasslands under drought conditions. Conversely, under surplus water conditions, transpiration and evaporation were higher from native short grass than from native tall tussock, suggesting a lower capacity of native tall tussock to remove surplus water [122].

The grazing method affects plant and litter cover, which in turn affect water dynamics through evapotranspiration fluxes. In the Flooding Pampa, litter cover is significantly increased by CG compared to continuous grazing in all the communities studied [48,51]. In the upland community, CG significantly increased the gravimetric water content [134]. In the halophyte steppe, the community with fewer resource, CG reduced bare soil from 71% under continuous grazing to 43%, due to a significant increase in plant cover [51]. Higher litter and plant cover in these halomorphic soils lead to a reduction in water loss by evaporation, which improves the hydric and thermal regime of the soil and thus reduces the risk of salinization [138]. This triggers a positive feedback loop between improvements in soil and plant condition.

3.4.4 Cultural Services

Cultural ecosystem services are defined as the multiple intangible values and non-material benefits provided by ecosystems through experiences, recreation and tourism that enhance human well-being by satisfying physiological, psychological and emotional needs [139]. Grasslands provide important cultural services such as cultural heritage, spiritual, aesthetic and social cohesion; tourism and recreation; education and scientific study [14]. However, relevant studies on cultural ecosystem services are relatively scarce and mainly cover Europe and North America [140].

Protected areas of varying conservation status have been established in the Flooding Pampa to ensure the long-term conservation of the grassland pampa biome and its cultural diversity. These public protected areas cover 32,534 ha and are visited for recreational activities such as bird watching and hiking, for educational purposes and for scientific studies [141]. However, only a very small percentage, 0.36%, of the grasslands in the Flooding Pampa are under public protection [73].

In recent years, the increasing preference of local tourists by natural and semi-natural environments for recreation has coincided with the interest of livestock farmers to conserve native grasslands under grazing for livestock production and, at the same time, to include rural tourism as an alternative source of economic income [142]. Several examples of livestock farms in the Flooding Pampa that have been incorporated into the Wildlife Refuge Network, conserve almost 8000 hectares of native animals and plants of the grassland communities [73,143].

Livestock farming in Pampa grassland is an ancient and deeply rooted cultural heritage, dating back to the origin of Argentina. The social cohesion around this activity and the traditional celebrations related to animal husbandry are relevant in each location of the Flooding Pampa. The traditional herding system for cattle keeping is an integral part of social cohesion in rural landscapes [144,145].

4  Conclusion

Anthropogenic interventions such as land use change, continuous grazing, and glyphosate spraying, as well as increased stocking rates, have caused severe degradation of the Flooding Pampa grasslands. Consequently, the ecosystem services provided by the grasslands are declining, including provisioning services (despite increased inputs, current meat production is low) and regulating and supporting services. The study of ecosystem services in this region has been primarily concerned with the impact of drivers on floristic and animal diversity as well as on primary production. There has been considerably less research conducted on other services, including climate regulation, water provision, nutrient cycling, meat production, and soil erosion. Furthermore, there is a significant lack of research on the cultural services that connect society and nature and promote a comprehensive public understanding and protection of ecosystems.

Controlled Grazing, AMPG’s system design for managing Flooding Pampa grasslands, and conservative stocking rates has been shown to reverse degradation and to make compatible production and economic profits with ecosystem conservation. Nevertheless, further research is required to ascertain the circumstances that impede the transmission of such insights to producers, with the objective of implementing them in practical applications. Moreover, future research directions should focus on the impact of livestock farming management practices, such as grazing methodology and stocking rate, as well as those applied to enhance grassland production or quality, such as fertilization, weed control, burning, interseeding, water systematization, and afforestation, on C sequestration, water dynamics, nutrient cycling, and plant and animal diversity. This research should be conducted at the farm-scale and over the long term.

Acknowledgement: The authors thank the anonymous reviewers, whose comments improved the quality of the paper.

Funding Statement: The authors received no specific funding for this study.

Author Contributions: Both authors confirm equal contribution to the paper: designed the literature search, wrote and organization of the manuscript. All authors reviewed the results and approved the final version of the manuscript.

Availability of Data and Materials: Not applicable.

Ethics Approval: Not applicable.

Conflicts of Interest: The authors declare that they have no conflicts of interest to report regarding the present study.

1We named “natural grassland” to those old-growth grasslands, which are ancient ecosystems characterized by high herbaceous species richness, high endemism, and unique species compositions, which biodiversity is maintained by frequent fires or grazing. While “seminatural grassland” are those “secondary grassland”, which are novel, anthropogenic young ecosystems derived from agriculture or other anthropogenic intervention that modified their original composition [9].

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