TY  - EJOU
AU  - FANG, YINCHUN 
AU  - LIU, XINHUA 
AU  - YANG, XU 
AU  - WANG, CUIE 

TI  - Covalent Immobilization of Lipase on Novel Nanofibrous Membrane for Catalysis of the Organic Synthesis
T2  - Journal of Polymer Materials

PY  - 2019
VL  - 36
IS  - 2
SN  - 0976-3449

AB  - Enzymes are green biocatalysts which have been widely used in many fields. Immobilization
enzymes on nanofibrous membrane possessed easy recycling and high stability which would
broaden their applications. Covalent immobilization of lipase could endow them higher stability
than other protocols. In this study, a novel nanofibrous membrane containing epoxy groups and
hydrophilic polyethylene oxide branch was used as a support for lipase immobilization. The
immobilized lipase was used as the biocatalyst to catalyse Rap. stroermer reaction. The results
showed that it obtained the high product yield of 88% when the volume ratio of methanol and
water was 4:1, the dosage of immobilized lipase was 40~50 mg, the reaction temperature and
time were 30~35 °C and 10 h.
KW  - Enzyme immobilization
KW  -  Lipase
KW  -  Electrospinning
KW  -  Biocatalyst
KW  -  Organic synthesis

DO  - 10.32381/JPM.2019.36.02.1