@Article{JPM.2021.38.3-4.4,
AUTHOR = {S.R. MANE, E. K. PATHAN, G. PATIL, S. G. TUPE, V. GHORMADE, B. P.
CHAUDHARI, M.V. DESHPANDE},
TITLE = {Taguchi Design of the Experimental approach to Increase the Biomass and Cell Wall Chitosan Contents of Zygomycetous Dimorphic Fungus <i>Benjaminiella Poitrasii</i>},
JOURNAL = {Journal of Polymer Materials},
VOLUME = {38},
YEAR = {2021},
NUMBER = {3-4},
PAGES = {219--230},
URL = {http://www.techscience.com/jpm/v38n3-4/56027},
ISSN = {0976-3449},
ABSTRACT = {A dimorphic fungus Benjaminiella poitrasii contains high chitin/chitosan (35% of the cell wall) in
the mycelial (M) form than its yeast (Y) form (20% of the cell wall). However, the relative proportion
of chitosan is more in yeast form cells (chitosan: chitin ratio, 6:1) than mycelial cells (chitosan:
chitin ratio, 3:1). Using the Taguchi design of experimental (DOE) approach, interactions among
eight different parameters showed that carbon source (starch, 10 g/L), incubation time (48 h),
inoculum (M and Y mixed 10%), yeast extract (6 g/L) and peptone (10 g/L), were optimum for
maximum biomass production. Under these conditions, the chitosan yield from the mycelia was
60.89±2.30 mg/g of dry biomass, while from the yeast cells was 28.29±2.7 mg/g. The molecular
weights of chitosan isolated from M and Y cells were 41.28 kDa and 21.72 kDa, respectively as
measured by gel permeation chromatography. The degree of deacetylation of chitosans as
88.17-90.72% DDA measured by <sup>1</sup>
H-NMR. Furthermore, chitosans from M and Y cells inhibited
the growth of plant pathogenic Fusarium oxysporum (MIC<sub>90</sub>0.1 and 0.4 mg/mL) and Ustilago
maydis (MIC<sub>90</sub>0.4 and 0.8 mg/mL) at lower concentrations as compared to chitosan isolated
from marine sources (MIC<sub>90</sub> 0.8 and >1.6 mg/mL).},
DOI = {10.32381/JPM.2021.38.3-4.4}
}