TY  - EJOU
AU  - KUMAR, PRABHAT 
AU  - SARKAR, ANJANA 
AU  - JAIN, PURNIMA 

TI  - Green Synthesis of Reduced Graphene Oxide Nanosheet by using L-ascorbic Acid and Study of its Cytotoxicity on Human Cervical Cancer Cell Line
T2  - Journal of Polymer Materials

PY  - 2022
VL  - 39
IS  - 1-2
SN  - 0976-3449

AB  - Biocompatible graphene derivative materials (GBMs) to harness the maximum potential of
pristine graphene biologically, is the most important strategy for its advanced applications in
pharmaceutical and other biomedical fields. Currently, scientists are trying to find this by using
biopolymer nanocomposites or anchored materials. Nevertheless, tuning the bare GBMs towards
biocompatibility is a beautiful approach to exploit the fundamental potential of pristine graphene
vis-à-vis suppressing the effects of incorporated biopolymers or anchored materials. Herein, a
large-scale, cost-effective, facile, and environment-friendly green synthetic strategy is used for
the synthesis of reduced graphene oxide (rGO) nanosheet using L-ascorbic acid (L-AA) as a
reducing/stabilizing/capping agent. The as-synthesized rGO was characterized by XRD, Raman
spectroscopy, TEM, and in-vitro cell cytotoxicity through SiHa (human cervical cancer) cell line.
Results showed that nanosheet of rGO was synthesized successfully and the order of cell
viability on SiHa cell line was found to be rGO <sub>ascorbic acid</sub>> GO > rGO<sub>hydrazine</sub>. The reason behind
such viability order may be surface oxidation state, carbon content, and presence of reducing/
surfactant/capping agent along with graphene. The studies described can be further optimized
to be used in neural tissue engineering, regenerative medicines, biosensors, drug delivery, and
gene delivery therapy, to name a few.
KW  - Reduced graphene oxide
KW  -  Nanosheet
KW  -  Ascorbic acid
KW  -  Cytotoxicity; SiHa Cell line
KW  -  Biomaterial

DO  - 10.32381/JPM.2022.39.1-2.8