TY - EJOU
AU - Wang, Xuesong
AU - Wang, Zhihong
AU - Yang, Qiuling
AU - Peng, Sheng
AU - Peng, Mijun
TI - Determining the in vitro Anti-Aging Effect of the Characteristic Components from Eucommia ulmoides
T2 - Journal of Renewable Materials
PY - 2022
VL - 10
IS - 12
SN - 2164-6341
AB - To evaluate the potential anti-aging ability of Eucommia ulmoides, four characteristic components (chlorogenic
acid, geniposidic acid, aucubin, quercetin) were selected to assess their effects on H2O2-induced oxidative damage
model of human umbilical vein endothelial cell (HUVEC). Oxidative damage indexes, inflammatory factors, cell
cycle, cell apoptosis, cell senescence, and their related proteins were analyzed by methyl thiazolyl tetrazolium
(MTT) assay, enzyme-linked immunosorbent assay (ELISA), propidium iodide (PI) staining, annexin V-FITC/
PI double staining, SA β-galactosidase staining, and western blotting (WB). The results showed that H2O2-
induced cell growth inhibition rate decreased as supplementation with characteristic components when compared
to H2O2 group. Meanwhile, the contents of antioxidant indexes (reactive oxygen species, lactate dehydrogenase,
molondialdehyde, superoxide dismutase, glutathione), inflammatory factors (nuclear factor kappa-B, intercellular
cell adhesion molecule-1, vascular cell adhesion protein 1), and functional factors (NO, Endothelin-1) in characteristic components treated groups improved if comparison with H2O2 group, suggesting the characteristic components of E. ulmoides could alleviate H2O2-induced oxidative damage. Moreover, cell cycle, cell apoptosis, cell
senescence, and their related proteins under characteristic components treatment exhibited a better effect than
under H2O2 treatment, implying the characteristic components could participate in anti-aging via multiple pathways. These results manifested that the characteristic components of E. ulmoides posses the capacity of anti-aging,
which provided a basis for investigating the anti-aging ability of E. ulmoides itself.
KW - Anti-aging; Eucommia ulmoides; characteristic components; oxidative damage; cell senescence
DO - 10.32604/jrm.2022.020204