TY  - EJOU
AU  - Volova, Tatiana 
AU  - Zhila, Natalia 
AU  - Sapozhnikova, Kristina 
AU  - Menshikova, Olga 
AU  - Kiselev, Evgeniy 
AU  - Sukovatyi, Alexey 
AU  - Volkov, Vladimir 
AU  - Peterson, Ivan 
AU  - Ipatova, Natalia 
AU  - Shishatskaya, Ekaterina 

TI  - From Waste to Biopolymer: Synthesis of P(3HB-<i>co</i>-4HB) from Renewable Fish Oil
T2  - Journal of Renewable Materials

PY  - 2025
VL  - 13
IS  - 3
SN  - 2164-6341

AB  - The article presents the results of a study on the possibility of synthesizing biodegradable poly(3-hydroxybutyrate-co-4-hydroxybutyrate) [P(3HB-<i>co</i>-4HB)] from renewable waste fish oil (WFO) by the <i>Cupriavidus necator</i> B-10646 bacterium. For the first time, waste oil generated during the processing of <i>Sprattus balticus</i> in the production of sprats was used as the main carbon substrate for the synthesis of P(3HB-<i>co</i>-4HB), and ε-caprolactone was used as a precursor instead of the more expensive γ-butyrolactone. Samples of P(3HB-<i>co</i>-4HB) with a 4HB monomer content from 7.4 to 11.6 mol.% were synthesized, and values of the bacterial biomass yield and the total yield of the copolymer were comparable with the control (where butyric acid was used as carbon source). The following properties of the samples were studied: molecular weight, temperature characteristics, thermal behavior, isothermal crystallization of melts, and the formation of spherulites. The renewable fatty substrate of complex composition was used to synthesize samples of technologically advanced low-crystallinity P(3HB-<i>co</i>-4HB) with significant proportions of 4HB, without impairing the physicochemical properties of the polymer. The biotechnological process involving the use of renewable WFO and ε-caprolactone can be employed to reduce the costs of producing a promising “green” bioplastic and make it more affordable.
KW  - Waste fish oil; biodegradable polyhydroxyalkanoates; renewable materials; biosynthesis; copolymers

DO  - 10.32604/jrm.2024.058775