TY  - EJOU
AU  - Alvarado-Cordero, Enzo 
AU  - Oca-Vásquez, Gabriela Montes de 
AU  - Pereira-Reyes, Reinaldo 
AU  - Vega-Baudrit, José 
AU  - Esquivel-Alfaro, Marianelly 

TI  - Production of Polyhydroxybutyrate (PHB) by Bacillus megaterium DSM 32 from Residual Glycerol of the Bioenergy Industry
T2  - Journal of Renewable Materials

PY  - 2017
VL  - 5
IS  - 3-4
SN  - 2164-6341

AB  - Biodegradable polymers from renewable resources are generating growing interest in the plastic industry because they have properties similar to synthetic polymers. Polyhydroxyalkanoates, mainly polyhydroxybutyrate (PHB), have mechanical and physicochemical properties very similar to their synthetic counterparts. This work explores the use of residual glycerol from the bioenergy industry for the production of PHB by Bacillus megaterium DSM 32. The glycerol works as a source of carbon and energy. Raw glycerol was purified with sulfuric acid in order to neutralize saponified fatty acids. The purification process generated three different phases. One of the phases was the glycerol-rich layer; this layer was filtered and concentrated by vacuum distillation process. The purity of the glycerol was determined by thermogravimetric analysis (TGA). Additionally, the physicochemical properties, like viscosity, pH, ash content and density, were measured. The experiments were conducted in shake flasks at 30°C and 120 rpm. Different glycerol concentrations (20, 30, 40 g/L) were used to evaluate the influence of the initial concentration of glycerol on the biomass accumulation and biopolymer production. The purified glycerol obtained had a high purity (~ 89.5–92.13%); this material does not contain fatty acids, although it contains ~3.7% salts. The final PHB concentration obtained was 0.054 mg/mL.
KW  - Polyhydroxybutyrate
KW  -  glycerol
KW  -  biopolymer
KW  -  Bacillus megaterium
KW  -  biomass

DO  - 10.7569/JRM.2017.634123