@Article{mcb.2017.014.081,
AUTHOR = {Yidan Chen, Kang Zhang, Juhui Qiu, Shicheng He, Junyang Huang, Lu Huang, Dongyu Jia, Bo Ling, Da Sun, Xiang Xie, Tieying Yin,  Guixue Wang},
TITLE = {Shear Stress-mediated Angiogenesis Through Id1 Relevant to Atherosclerosis},
JOURNAL = {Molecular \& Cellular Biomechanics},
VOLUME = {14},
YEAR = {2017},
NUMBER = {2},
PAGES = {83--100},
URL = {http://www.techscience.com/mcb/v14n2/28600},
ISSN = {1556-5300},
ABSTRACT = {Abnormal shear stress in the blood vessel is an important stimulating factor for the formation of angiogenesis and vulnerable plaques. This paper intended to explore the role of shear stress-regulated Id1 in angiogenesis. First, we applied a carotid artery ring ligation to create local stenosis in ApoE<sup>-/-</sup> mice. Then, 3D geometry of the vessel network was reconstructed based on MRI, and our analysis of computational fluid dynamics revealed that wall shear stress of the proximal region was much higher than that of the distal region. In addition, results from histological staining of the proximal region found more vulnerable-probe plaques with new capillary formation, the presence of macrophages and collagen fibers degradation. Our in <i>vitro</i> and in <i>vivo</i> experiments further indicated high shear stress can induce endothelial cell-mediated angiogenesis and high expression of Id1. Id1-overexpression promoted endothelial cells migration and angiogenesis through collagen degradation mediated by MT-MMPs. Together, our results support a biomechanical role for Id1 in angiogenesis, suggesting manipulation of the Id1 activity may offer a novel anti-angiogenic therapeutic strategy in vulnerable plaques.},
DOI = {10.3970/mcb.2017.014.081}
}