TY  - EJOU
AU  - Liu, Kefeng  
AU  - Zhang, Zhengyu  
AU  - Pei, Ting  
AU  - Li, Ziqing  
AU  - Wang, Jingjing  
AU  - Wang, Hong  
AU  - Ping, Suning  
AU  - Deng, Lie  
AU  - Wang, Linli  
AU  - Huang, Jintao  
AU  - Sheng, Puyi 
AU  - Liu, Shuying 
AU  - Li, Chaohong 

TI  - Simvastatin Inhibits the Proliferation and Apoptosis of Macrophages Induced by Mechanical and/or Oxidized Low-Density Lipoprotein
T2  - Molecular \& Cellular Biomechanics

PY  - 2017
VL  - 14
IS  - 2
SN  - 1556-5300

AB  - This study was designed to investigate the effects of mechanical (MS) and/or oxidized low-density lipoprotein on proliferation and apoptosis of RAW264.7 macrophages and the underlying mechanisms. The cultured quiescent RAW264.7 macrophages were subject to stimulation with MS and/or in the presence or absence of simvastatin and then harvested for Western blot, and immunoflourecence. Either MS or alone could cause increase in cell proliferation and apoptosis, while their combination led to an additive effect. In terms of mechanisms, MS and/or significantly increased phosphorylation levels of MAPKs (ERKs, JNKs and p38MAPK), promoted the reactive oxygen species (ROS) and up-regulated DNA methylation in RAW264.7 macrophages. The increased DNA methylation was associated with proliferation but not apoptosis. In contrast, simvastatin could remarkably inhibit all the effects mentioned above. MS and can simultaneously promote both proliferation and apoptosis of macrophages through activating MAPKs, ROS, and DNA methylation signaling, which can be directly inhibited by the simvastatin treatment. The study results can provide novel information for the pathogenesis and prevention of hypertensive mechanical related vascular diseases.
KW  - Oxidative stress
KW  -  apoptosis
KW  -  macrophages

DO  - 10.3970/mcb.2017.014.099