TY  - EJOU
AU  - Sapkal, Pranav S.  
AU  - Kuthe, Abhaykumar M.  
AU  - Mathankar, Shantanu  
AU  - Deshmukh, Akash A.  

TI  - 3D Bio-Plotted Tricalcium Phosphate/Zirconia Composite Scaffolds to Heal Large Size Bone Defects
T2  - Molecular \& Cellular Biomechanics

PY  - 2017
VL  - 14
IS  - 2
SN  - 1556-5300

AB  - β-TCP-Zirconia scaffolds with different architectures were fabricated by means of 3D-Bioplotting in order to enhance the mechanical and in-vitro ability of the scaffold to heal large size bone defects. In the present study scaffold architecture with different strand orientations (0°-90°, 0°-45°-135°-180°, 0°-108°-216° and 0°-72°-144°-36°-108°) were fabricated, characterized and evaluated for mechanical strength and cell proliferation ability. β-TCP powder (25 µm) and PVA (Polyvinyl Alcohol) was acquired from Fisher Scientific, India. Zirconia (18 to 32 µm) was procured from Lobachemie, India. In brief 7.5%, PVA in distilled water was used as a binder and was mixed with 10 grams of (70/30) TCP-Zirconia ratio to make the ceramic paste. The paste was further sieved through a 100-micron sieve and was filled in a 30 ml syringe. With 400 microns needle, the scaffold architectures were printed layer by layer and were allowed to dry at room temperature. The dried samples were sintered at 1500oC in a silicon carbide furnace and were allowed to remain at this temperature for 5 hours. The sintered samples were then characterized by X-Ray Diffraction, Scanning Electron Microscopy, Uniaxial Compression Tests, Fourier transform infrared spectroscopy and cell proliferation by XTT assay using MG-63 human osteosarcoma cell line. It was revealed that all samples maintained their structure and functional groups after sintering. Also, it was found that the architecture with (0°-72°-144°-36°-108°) strand orientation had the best strength and cell proliferation ability. Jointly these properties are required for scaffold fabrication in the field of bone tissue engineering.
KW  - 3D-Bioplotter
KW  -  in-vitro
KW  -  β-TCP
KW  -  Zirconia & Bone Tissue Engineering

DO  - 10.3970/mcb.2017.014.123