@Article{mcb.2019.07074,
AUTHOR = {Qi Dang, Chun-Gong Li, Xin-Xin Jin, Ya-Jin Zhao, Xiang Wang},
TITLE = {Microspheres Modified with the Heparin Increasing the Length of Molecular Linker to Better Capture the Endotoxin},
JOURNAL = {Molecular \& Cellular Biomechanics},
VOLUME = {16},
YEAR = {2019},
NUMBER = {Suppl.2},
PAGES = {146--146},
URL = {http://www.techscience.com/mcb/v16nSuppl.2/35255},
ISSN = {1556-5300},
ABSTRACT = {Endotoxin is a a very powerful and toxic inflammatory stimulator usually leading to the sepsis occurred. In order to remove endotoxin better through hemoperfusion, it is a pretty choice to increase the length of molecular linker on adsorbents. In this study, we chose the heparin as a molecular linker because of its being anticoagulant linear polysaccharide. Heparin as a linker was covalently immobilized on the chloromethylated polystyrene microspheres (Ps) and then connected with L-phenylalanine (Phe) forming the Ps-Hep-Phe structure to adsorbed endotoxin better. The property of microspheres was characterized by Fourier transform infrared spectroscopy, X-ray photoelectron spectroscopy, zeta potential and water contact angle. The hydrophilicity was improved after immobilization. The adsorption capacity of Ps-Hep-Phe for endotoxin adsorption was higher than that of Ps-Phe (No heparin). And the adsorbents with the heparin as a linker simultaneously showed the prolonged clotting times, low protein adsorption, and reduced the hemolysis rate, indicating that heparin as a molecular linker could play an important role in anticoagulation. Therefore, this study implied that heparin would be a promising strategy for adsorbents modification in hemoperfusion. },
DOI = {10.32604/mcb.2019.07074}
}