@Article{oncologie.2020.012493,
AUTHOR = {Hui Zhang, Zhan Wang, Lin Liu, Fuqiang Zhang, Yuxin Song, Yaowen Qian},
TITLE = {Expression Changes, Prognostic Analysis and Risk Factors of miR-625-3p and  miR-449a in Osteosarcoma Patients after Surgery},
JOURNAL = {Oncologie},
VOLUME = {22},
YEAR = {2020},
NUMBER = {1},
PAGES = {23--33},
URL = {http://www.techscience.com/oncologie/v22n1/39628},
ISSN = {1765-2839},
ABSTRACT = {The expression changes of miR-625-3p and miR-449a in osteosarcoma 
patients after surgery is the critical study in this paper. Analysis of their prognosis 
and risk factors can be helpful in understanding the prognostic value for clinical 
purposes. Fifty-eight patients with osteosarcoma diagnosed in our hospital were 
considered as the research group (RG), and 52 health subjects at the same time 
were collected as the control group (CG). Fluorescence quantitative PCR (RTPCR) was employed to test the expression levels of miR-625-3p and miR-449a in 
the serum of subjects in both groups before surgery and patients in the RG after 
surgery. The survival conditions of both groups with high and low miR-625-3p 
and miR-449a expression levels were compared after 5-year follow-up of patients 
in the RG. The prognostic factors of osteosarcoma patients were assessed through 
Cox regression analysis. The miR-625-3p expression level in the RG was 
dramatically higher than that in the CG (<i>p</i> < 0.05), and the miR-449a expression 
level was dramatically lower than that in the CG (<i>p</i> < 0.05). The miR-625-3p 
expression level in the RG after surgery was markedly lower than that before 
surgery (<i>p</i> < 0.05), and the miR-449a expression level was markedly higher than 
that before surgery (<i>p</i> < 0.05). After they were followed up for 5 years, the overall 
survival rate of those in the RG was 60.34%, the rate of miR-625-3p low 
expression group (LEG) was dramatically higher than that of high expression 
group (HEG) (<i>p</i> < 0.05), and the rate of miR-449a HEG was markedly higher than 
that of miR-449a LEG (<i>p</i> < 0.05). The relative expression of miR-625-3p and miR-
449a was remarkably different from tumor size, lymph node metastasis, and 
Enneking stage (<i>p</i> < 0.05). Univariate Cox regression analysis identified that tumor 
size, lymph node metastasis, Enneking stage, miR-625-3p and miR-449a were risk 
factors affecting the prognosis of patients; multivariate Cox regression analysis 
found that the five were independent prognostic factors of them. miR-625-3p and 
miR-449a are differentially expressed in the postoperative serum of osteosarcoma 
patients, and their differential expression is an independent risk factor affecting 
their prognosis, which has prognostic value and can provide a reference value for 
clinical practice.},
DOI = {10.32604/oncologie.2020.012493}
}